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| Year/Semester of Study | 3 / Spring Semester | ||||
| Level of Course | 1st Cycle Degree Programme | ||||
| Type of Course | Compulsory | ||||
| Department | BIOLOGY | ||||
| Pre-requisities and Co-requisites | None | ||||
| Mode of Delivery | Face to Face | ||||
| Teaching Period | 14 Weeks | ||||
| Name of Lecturer | SEVİL SUNGUR (sevilsungur@nevsehir.edu.tr) | ||||
| Name of Lecturer(s) | |||||
| Language of Instruction | Turkish | ||||
| Work Placement(s) | None | ||||
| Objectives of the Course | |||||
| Molecular techniques to teach | |||||
| Learning Outcomes | PO | MME | |
| The students who succeeded in this course: | |||
| LO-1 | Using molecular biological techniques to solve problems |
PO-1 Understand the basic concepts regarding the biology programs PO-5 Are dedicated to improve themselves by keeping abreast of the developments in science, technology and modern topics PO-9 Are able to express themselves in verbal and written communication having the ability to work individually, take initiatives and make their own decisions PO-14 Are able to use the information and communication technology required by the field of Biology with a competence at the level of European Computer Driving License |
Examination |
| LO-2 | Knows moleculer techniques used at medicine and biotechnology |
PO-7 Are able to use the modern techniques and devices necessary for the current practices in the field of Biology |
Examination |
| PO: Programme Outcomes MME:Method of measurement & Evaluation |
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| Course Contents | ||
| Kromosomal and Plasmid-DNA isolation of Bacteria and other microorganisms, Agarose gel electrophoresis Protein isolation SDS-PAGE, Transfer of genetic material in bacteria (transformation), Transfer of genetic material in bacteria (transformation), | ||
| Weekly Course Content | ||
| Week | Subject | Learning Activities and Teaching Methods |
| 1 | Kromosomal and Plasmid-DNA isolation of Bacteria and other microorganisms | Experimentation |
| 2 | Agarose gel electrophoresis | Experimentation |
| 3 | Protein isolation | Experimentation |
| 4 | SDS-PAGE, | Experimentation |
| 5 | Transfer of genetic material in bacteria (transformation), | Experimentation |
| 6 | Transfer of genetic material in bacteria (transformation), | Experimentation |
| 7 | Mutagens Implementation | Experimentation |
| 8 | mid-term exam | |
| 9 | Some bacteria and other microorganisms on Mutagenlerin Chemical Application | Experimentation |
| 10 | Some bacteria and other microorganisms on Mutagenlerin Chemical Application | Experimentation |
| 11 | Determination of Degree of Purity and quantity of DNA | Experimentation |
| 12 | Polymerase chain reaction | Experimentation |
| 13 | Polymerase chain reaction | Experimentation |
| 14 | Enzymatic Restriction endonuclease cutting of DNA and Application of Enzymes | Experimentation |
| 15 | Enzymatic Restriction endonuclease cutting of DNA and Application of Enzymes | Experimentation |
| 16 | final exam | |
| Recommend Course Book / Supplementary Book/Reading | ||
| Required Course instruments and materials | ||
| Freezer, autoclave, Electrophoresis System, vortex, gel imaging system (UVP), centrifuge device, laboratory consumables and chemicals. | ||
| Assessment Methods | |||
| Type of Assessment | Week | Hours | Weight(%) |
| mid-term exam | 8 | 2 | 40 |
| Other assessment methods | |||
| 1.Oral Examination | |||
| 2.Quiz | |||
| 3.Laboratory exam | |||
| 4.Presentation | |||
| 5.Report | |||
| 6.Workshop | |||
| 7.Performance Project | |||
| 8.Term Paper | |||
| 9.Project | |||
| final exam | 16 | 2 | 60 |
| Student Work Load | |||
| Type of Work | Weekly Hours | Number of Weeks | Work Load |
| Weekly Course Hours (Theoretical+Practice) | 2 | 14 | 28 |
| Outside Class | |||
| a) Reading | 0 | ||
| b) Search in internet/Library | 4 | 1 | 4 |
| c) Performance Project | 0 | ||
| d) Prepare a workshop/Presentation/Report | 0 | ||
| e) Term paper/Project | 0 | ||
| Oral Examination | 0 | ||
| Quiz | 0 | ||
| Laboratory exam | 0 | ||
| Own study for mid-term exam | 3 | 2 | 6 |
| mid-term exam | 2 | 1 | 2 |
| Own study for final exam | 2 | 9 | 18 |
| final exam | 2 | 1 | 2 |
| 0 | |||
| 0 | |||
| Total work load; | 60 | ||