Nevşehir Hacı Bektaş Veli University Course Catalogue
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| Year/Semester of Study | 3 / Fall Semester | ||||
| Level of Course | 3rd Cycle Degree Programme | ||||
| Type of Course | Compulsory | ||||
| Department | MOLECULAR BIOLOGY AND GENETICS | ||||
| Pre-requisities and Co-requisites | None | ||||
| Mode of Delivery | Face to Face | ||||
| Teaching Period | 14 Weeks | ||||
| Name of Lecturer | ENVER ERSOY ANDEDEN (enverandeden@nevsehir.edu.tr) | ||||
| Name of Lecturer(s) | |||||
| Language of Instruction | Turkish | ||||
| Work Placement(s) | None | ||||
| Objectives of the Course | |||||
| Aim of the course is to provide the students with basic experience in a wide variety of biochemical research techniques, and perform chemical and enzymatic analysis using calorimetric, electrophoretic, spectrophotometric and chromatographic techniques. | |||||
| Learning Outcomes | PO | MME | |
| The students who succeeded in this course: | |||
| LO-1 | can use laboratory instruments and work with chemicals |
PO-1 Obtain basic knowledge of Biology PO-7 Work effectively in multi-disciplinary research teams PO-9 Find out new methods to improve his/her knowledge PO-12 Demonstrate professional and ethical responsibility PO-15 Have contributed to scientific advances with research and development abilities PO-16 Explain the scientific findings clearly and efficiently both in written and verbal communications. PO-17 Survey, analyze and interpret the experimental results and to reach a scientific result using the available scientific data. |
Laboratory Exam |
| LO-2 | can apply chromatographic techniques to purify proteins. |
PO-7 Work effectively in multi-disciplinary research teams PO-8 Develop an awareness of continuous learning in relation with modern technology PO-10 Demonstrate sufficiency in English to follow literature, present technical projects and write articles PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics PO-14 Have knowledge about the main research interests of the molecular biology and genetics |
Laboratory Exam |
| LO-3 | can characterize the purified enzymes. |
PO-1 Obtain basic knowledge of Biology PO-7 Work effectively in multi-disciplinary research teams PO-8 Develop an awareness of continuous learning in relation with modern technology PO-11 Apply biological concepts to personal, social, economical, technological and ethical issues PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics PO-14 Have knowledge about the main research interests of the molecular biology and genetics |
Laboratory Exam |
| LO-4 | can apply spectroscopic, SDS-PAGE, and Western Blotting techniques for different protein labours. |
PO-7 Work effectively in multi-disciplinary research teams PO-8 Develop an awareness of continuous learning in relation with modern technology PO-9 Find out new methods to improve his/her knowledge |
Laboratory Exam |
| PO: Programme Outcomes MME:Method of measurement & Evaluation |
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| Course Contents | ||
| Acid and bases, preparation of buffers, Carbohydrate fermantation, Spectrophotometric analysis, Isolation of biomolecules, Protein purification, LDH purification, determination of protein quantity, calculations, Characterization of LDH, via SDS-PAGE and Western Blotting | ||
| Weekly Course Content | ||
| Week | Subject | Learning Activities and Teaching Methods |
| 1 | Introduction, Biochemistry Lab Rules,Details about Lab notebook and Lab report | Explanation, Experimentation |
| 2 | Analysis of experimental data, usage of micro pipette, preparation of biochemical solutions | Explanation, Experimentation |
| 3 | Acid and bases, preparation of buffers | Explanation, Experimentation |
| 4 | Carbohydrate fermantation with baking yeast | Explanation, Experimentation |
| 5 | Spectrophotometry, Spectroscopy and dilutions, Analysis of results | Explanation, Experimentation |
| 6 | Cell extract preparation | Explanation, Experimentation |
| 7 | Isolation of biomolecules | Explanation, Experimentation |
| 8 | mid-term exam | |
| 9 | I. Protein purification LDH purification | Explanation, Experimentation |
| 10 | II. Protein purification LDH purification | Explanation, Experimentation |
| 11 | III. Protein purification, determination of LDH enzyme activity | Explanation, Experimentation |
| 12 | IV. Protein purification determination of protein quantity, calculations, short ways | Explanation, Experimentation |
| 13 | V. Characterization of LDH, SDS-PAGE | Explanation, Experimentation |
| 14 | VI. Characterization of LDH, Western Blotting | Explanation, Experimentation |
| 15 | VII. Characterization of LDH, Western Blotting | Explanation, Experimentation |
| 16 | final exam | |
| Recommend Course Book / Supplementary Book/Reading | ||
| 1 | Nelson DL ve Cox MM, 2008. Lehninger Principles of Biochemistry (5. basım), WH Freeman | |
| 2 | Laboratuvar Föyü | |
| Required Course instruments and materials | ||
| Laboratory catalogs, Lecture Notes, basic laboratory material | ||
| Assessment Methods | |||
| Type of Assessment | Week | Hours | Weight(%) |
| mid-term exam | 8 | 2 | 40 |
| Other assessment methods | |||
| 1.Oral Examination | |||
| 2.Quiz | |||
| 3.Laboratory exam | |||
| 4.Presentation | |||
| 5.Report | |||
| 6.Workshop | |||
| 7.Performance Project | |||
| 8.Term Paper | |||
| 9.Project | |||
| final exam | 16 | 2 | 60 |
| Student Work Load | |||
| Type of Work | Weekly Hours | Number of Weeks | Work Load |
| Weekly Course Hours (Theoretical+Practice) | 2 | 14 | 28 |
| Outside Class | |||
| a) Reading | 1 | 14 | 14 |
| b) Search in internet/Library | 1 | 14 | 14 |
| c) Performance Project | 0 | ||
| d) Prepare a workshop/Presentation/Report | 0 | ||
| e) Term paper/Project | 0 | ||
| Oral Examination | 0 | ||
| Quiz | 0 | ||
| Laboratory exam | 0 | ||
| Own study for mid-term exam | 2 | 7 | 14 |
| mid-term exam | 2 | 1 | 2 |
| Own study for final exam | 2 | 8 | 16 |
| final exam | 2 | 1 | 2 |
| 0 | |||
| 0 | |||
| Total work load; | 90 | ||