Nevşehir Hacı Bektaş Veli University Course Catalogue

Information Of Programmes

FACULTY OF LETTERS & SCIENCE / MBG 309 - MOLECULAR BIOLOGY AND GENETICS

Code: MBG 309 Course Title: MICROBIOLOGY LABORATORY Theoretical+Practice: 0+2 ECTS: 3
Year/Semester of Study 3 / Fall Semester
Level of Course 1st Cycle Degree Programme
Type of Course Compulsory
Department MOLECULAR BIOLOGY AND GENETICS
Pre-requisities and Co-requisites None
Mode of Delivery Face to Face
Teaching Period 14 Weeks
Name of Lecturer MUSA KAR (musa.kar@nevsehir.edu.tr)
Name of Lecturer(s)
Language of Instruction Turkish
Work Placement(s) None
Objectives of the Course
The aim of this lecture is to learn examination of microorganisms under the microscope, inoculation of microorganism in favorable mediums, identification and purification of microorganisms.

Learning Outcomes PO MME
The students who succeeded in this course:
LO-1 Can perform medium preparation and cell inoculation for bacteria. PO-1 Obtain basic knowledge of Biology
PO-2 Develop an understanding of matter and energy in organisms
PO-3 Identify structure-function relationships in cells and organisms
PO-4 Define the relationship among life forms and their environments and ecosystems
PO-10 Demonstrate sufficiency in English to follow literature, present technical projects and write articles
PO-11 Apply biological concepts to personal, social, economical, technological and ethical issues
PO-12 Demonstrate professional and ethical responsibility
Laboratory Exam
LO-2 Can perform gram staining. PO-6 Develop an understanding of nature and history of scientific thought
PO-7 Work effectively in multi-disciplinary research teams
PO-8 Develop an awareness of continuous learning in relation with modern technology
PO-9 Find out new methods to improve his/her knowledge
PO-10 Demonstrate sufficiency in English to follow literature, present technical projects and write articles
Laboratory Exam
LO-3 Can know microscopy of bacteria, cyanobacter and yeast. PO-4 Define the relationship among life forms and their environments and ecosystems
PO-6 Develop an understanding of nature and history of scientific thought
PO-7 Work effectively in multi-disciplinary research teams
PO-8 Develop an awareness of continuous learning in relation with modern technology
PO-9 Find out new methods to improve his/her knowledge
PO-10 Demonstrate sufficiency in English to follow literature, present technical projects and write articles
PO-11 Apply biological concepts to personal, social, economical, technological and ethical issues
Laboratory Exam
LO-4 Can obtain pure culture for bacteria. PO-1 Obtain basic knowledge of Biology
PO-2 Develop an understanding of matter and energy in organisms
PO-3 Identify structure-function relationships in cells and organisms
PO-4 Define the relationship among life forms and their environments and ecosystems
PO-6 Develop an understanding of nature and history of scientific thought
PO-7 Work effectively in multi-disciplinary research teams
Laboratory Exam
LO-5 Can perform antimicrobial tests. PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics
PO-14 Have knowledge about the main research interests of the molecular biology and genetics
PO-15 Have contributed to scientific advances with research and development abilities
PO-16 Explain the scientific findings clearly and efficiently both in written and verbal communications.
PO-17 Survey, analyze and interpret the experimental results and to reach a scientific result using the available scientific data.
Laboratory Exam
PO: Programme Outcomes
MME:Method of measurement & Evaluation

Course Contents
Introduction to microbiology laboratory, medium preparation for bacteria and yeast, selective medium preparation, inoculation and purification of bacteria, medium preparation for cyanobacter and inoculation, microscopy of bacteria, algea, yeast and fungi, antimicrobial applications
Weekly Course Content
Week Subject Learning Activities and Teaching Methods
1 Introduction to microbiology laboratory Explaining method, Experimentation
2 Medium preparation for bacteria and yeast Explaining method, Experimentation
3 Growing conditions of microorganisms Explaining method, Experimentation
4 Microscopy of bacteri and yeast Explaining method, Experimentation
5 Techniques of sampling and inoculation Explaining method, Experimentation
6 Methods of obtaining pure culture Explaining method, Experimentation
7 Conservation of pure culture and activation Explaining method, Experimentation
8 mid-term exam
9 Gram stain technique in bacteria Explaining method, Experimentation
10 Microscopy of cyanobacteria Explaining method, Experimentation
11 Preparation liquid and solid medium for cyanobacteria Explaining method, Experimentation
12 Test materials (blood, cerebrospinal fluid, urine, saliva) Explaining method, Experimentation
13 Counting microorganisms Explaining method, Experimentation
14 Detection enzyme activity in microorganisms Explaining method, Experimentation
15 Antimicrobial applications Explaining method, Experimentation
16 final exam
Recommend Course Book / Supplementary Book/Reading
Required Course instruments and materials
Lecture note

Assessment Methods
Type of Assessment Week Hours Weight(%)
mid-term exam 8 2 30
Other assessment methods
1.Oral Examination
2.Quiz
3.Laboratory exam 12 1 20
4.Presentation
5.Report 15 2 20
6.Workshop
7.Performance Project
8.Term Paper
9.Project
final exam 16 2 30

Student Work Load
Type of Work Weekly Hours Number of Weeks Work Load
Weekly Course Hours (Theoretical+Practice) 2 14 28
Outside Class
       a) Reading 1 7 7
       b) Search in internet/Library 1 7 7
       c) Performance Project 0
       d) Prepare a workshop/Presentation/Report 1 14 14
       e) Term paper/Project 0
Oral Examination 0
Quiz 0
Laboratory exam 1 7 7
Own study for mid-term exam 1 7 7
mid-term exam 2 1 2
Own study for final exam 2 8 16
final exam 2 1 2
0
0
Total work load; 90