Nevşehir Hacı Bektaş Veli University Course Catalogue
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| Year/Semester of Study | 2 / Spring Semester | ||||
| Level of Course | 3rd Cycle Degree Programme | ||||
| Type of Course | Compulsory | ||||
| Department | MOLECULAR BIOLOGY AND GENETICS | ||||
| Pre-requisities and Co-requisites | None | ||||
| Mode of Delivery | Face to Face | ||||
| Teaching Period | 14 Weeks | ||||
| Name of Lecturer | ENVER ERSOY ANDEDEN (enverandeden@nevsehir.edu.tr) | ||||
| Name of Lecturer(s) | |||||
| Language of Instruction | Turkish | ||||
| Work Placement(s) | None | ||||
| Objectives of the Course | |||||
| Aim of the course is to teach basic techniques used in molecular biology and recombinant DNA methods with applications. | |||||
| Learning Outcomes | PO | MME | |
| The students who succeeded in this course: | |||
| LO-1 | can perform restriction and DNA sequencing analysis. |
PO-5 Explain transmission of genetic background in organisms and populations PO-6 Develop an understanding of nature and history of scientific thought PO-8 Develop an awareness of continuous learning in relation with modern technology |
Examination |
| LO-2 | can isolate and purify proteins from organisms |
PO-5 Explain transmission of genetic background in organisms and populations PO-7 Work effectively in multi-disciplinary research teams PO-9 Find out new methods to improve his/her knowledge |
Examination |
| LO-3 | can have a detailed view about several gel electrophoresis and chromatography and perform experiments with those techniques. |
PO-16 Explain the scientific findings clearly and efficiently both in written and verbal communications. PO-17 Survey, analyze and interpret the experimental results and to reach a scientific result using the available scientific data. |
Examination |
| PO: Programme Outcomes MME:Method of measurement & Evaluation |
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| Course Contents | ||
| In situ hybridization techniques for DNA, DNA sequencing, Reporter gene construction, reporter assays, Protein isolation and purification, SDS-PAGE, Bradford Protein Assay, ELISA, Thin Layer and Column Chromatography | ||
| Weekly Course Content | ||
| Week | Subject | Learning Activities and Teaching Methods |
| 1 | In situ hybridization techniques for DNA; Southern and Northern Blotting, DNA-microchips | Explanation, Experimentation |
| 2 | Molecular marker techniques | Explanation, Experimentation |
| 3 | DNA sequencing; Sanger and Maxam-Gilbert techniques and new approaches | Explanation, Experimentation |
| 4 | DNA sequencing; STR (Short tandem repeats), SNP detection by RFLP (Restriction fragment length polymorphism) method | Explanation, Experimentation |
| 5 | DNA sequencing; AFLP (Amplified fragment length polymorphism) and ARDRA (Amplified Ribosomal DNA Restriction Analysis) | Explanation, Experimentation |
| 6 | Reporter gene construction, reporter assays I | Explanation, Experimentation |
| 7 | Reporter gene construction, reporter assays II | Explanation, Experimentation |
| 8 | mid-term exam | |
| 9 | Protein isolation and purification from bacterial cells | Explanation, Experimentation |
| 10 | SDS-PAGE for protein separation | Explanation, Experimentation |
| 11 | Bradford Protein Assay | Explanation, Experimentation |
| 12 | ELISA, Enzyme-Linked ImmunoSorbent Assay | Explanation, Experimentation |
| 13 | Thin Layer Chromatography | Explanation, Experimentation |
| 14 | Column Chromatography | Explanation, Experimentation |
| 15 | Western Blotting | Explanation, Experimentation |
| 16 | final exam | |
| Recommend Course Book / Supplementary Book/Reading | ||
| 1 | Watson J. ve diğerleri, 2008. Molecular Biology of the Gene, (7. baskı). San Francisco Pearson yayınları | |
| 2 | Davis LG, Dibner MD, Battey JF. 1986. Basic Methods in Molecular Biology. Elsevier | |
| Required Course instruments and materials | ||
| Laboratory Catalog, Lecture Notes, basic laboratory material | ||
| Assessment Methods | |||
| Type of Assessment | Week | Hours | Weight(%) |
| mid-term exam | 8 | 2 | 40 |
| Other assessment methods | |||
| 1.Oral Examination | |||
| 2.Quiz | |||
| 3.Laboratory exam | |||
| 4.Presentation | |||
| 5.Report | |||
| 6.Workshop | |||
| 7.Performance Project | |||
| 8.Term Paper | |||
| 9.Project | |||
| final exam | 16 | 2 | 60 |
| Student Work Load | |||
| Type of Work | Weekly Hours | Number of Weeks | Work Load |
| Weekly Course Hours (Theoretical+Practice) | 4 | 14 | 56 |
| Outside Class | |||
| a) Reading | 1 | 14 | 14 |
| b) Search in internet/Library | 1 | 14 | 14 |
| c) Performance Project | 0 | ||
| d) Prepare a workshop/Presentation/Report | 0 | ||
| e) Term paper/Project | 0 | ||
| Oral Examination | 0 | ||
| Quiz | 0 | ||
| Laboratory exam | 0 | ||
| Own study for mid-term exam | 2 | 8 | 16 |
| mid-term exam | 2 | 1 | 2 |
| Own study for final exam | 2 | 8 | 16 |
| final exam | 2 | 1 | 2 |
| 0 | |||
| 0 | |||
| Total work load; | 120 | ||