Nevşehir Hacı Bektaş Veli University Course Catalogue
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| Year/Semester of Study | 4 / Fall Semester | ||||
| Level of Course | 1st Cycle Degree Programme | ||||
| Type of Course | Optional | ||||
| Department | MOLECULAR BIOLOGY AND GENETICS | ||||
| Pre-requisities and Co-requisites | None | ||||
| Mode of Delivery | Face to Face | ||||
| Teaching Period | 14 Weeks | ||||
| Name of Lecturer | ÜMİT KUMBIÇAK (umitkumbicak@nevsehir.edu.tr) | ||||
| Name of Lecturer(s) | |||||
| Language of Instruction | Turkish | ||||
| Work Placement(s) | None | ||||
| Objectives of the Course | |||||
| The aim of this lecture, to teach the basic techniques and analysis used in Recombinant DNA Technology. | |||||
| Learning Outcomes | PO | MME | |
| The students who succeeded in this course: | |||
| LO-1 | Can explain the vectors used in Recombinant DNA technology . |
PO-1 Obtain basic knowledge of Biology PO-2 Develop an understanding of matter and energy in organisms PO-3 Identify structure-function relationships in cells and organisms PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics PO-14 Have knowledge about the main research interests of the molecular biology and genetics PO-15 Have contributed to scientific advances with research and development abilities |
Examination |
| LO-2 | Can known the clonal strategies for gene. |
PO-1 Obtain basic knowledge of Biology PO-2 Develop an understanding of matter and energy in organisms PO-3 Identify structure-function relationships in cells and organisms PO-9 Find out new methods to improve his/her knowledge PO-10 Demonstrate sufficiency in English to follow literature, present technical projects and write articles PO-11 Apply biological concepts to personal, social, economical, technological and ethical issues PO-15 Have contributed to scientific advances with research and development abilities |
Examination |
| LO-3 | Can known the blotting techniques. |
PO-1 Obtain basic knowledge of Biology PO-9 Find out new methods to improve his/her knowledge PO-10 Demonstrate sufficiency in English to follow literature, present technical projects and write articles PO-11 Apply biological concepts to personal, social, economical, technological and ethical issues PO-12 Demonstrate professional and ethical responsibility PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics PO-14 Have knowledge about the main research interests of the molecular biology and genetics |
Examination |
| PO: Programme Outcomes MME:Method of measurement & Evaluation |
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| Course Contents | ||
| Weekly Course Content | ||
| Week | Subject | Learning Activities and Teaching Methods |
| 1 | Introduction to Recombinant DNA Technologies | Explaining, Question and answer teaching |
| 2 | Gene Cloning | Explaining, Question and answer teaching |
| 3 | Vectors; Plasmids, Bacteriophage, Cosmids, M13 vectors | Explaining, Question and answer teaching |
| 4 | Gene expression vectors; Yeast vectors; Mammalian cell vectors; Super vectors (BAC, YAC, MAC, HAC) | Explaining, Question and answer teaching |
| 5 | Gene Transfer Techniques | Explaining, Question and answer teaching |
| 6 | Strategies for gene cloning | Explaining, Question and answer teaching |
| 7 | DNA analysis techniques | Explaining, Question and answer teaching |
| 8 | mid-term exam | |
| 9 | DNA isolation, Polymerase Chain Reaction (PCR) and electrophoresis | Explaining, Question and answer teaching |
| 10 | Southern Blotting, Northern Blotting, Western Blotting | Explaining, Question and answer teaching |
| 11 | Production of recombinant proteins | Explaining, Question and answer teaching |
| 12 | Making Recombinant DNA | Explaining, Question and answer teaching |
| 13 | Construction of genomic DNA and cDNA libraries | Explaining, Question and answer teaching |
| 14 | Gene therapy | Explaining, Question and answer teaching |
| 15 | Outputs of Recombinant DNA technologies | Explaining, Question and answer teaching |
| 16 | final exam | |
| Recommend Course Book / Supplementary Book/Reading | ||
| 1 | Molecular Biotechnology: Principles and Applications of Recombinant DNA. Bernard R. Glick , Jack J. Pasternak, Cheryl L. Patten | |
| Required Course instruments and materials | ||
| Textbook | ||
| Assessment Methods | |||
| Type of Assessment | Week | Hours | Weight(%) |
| mid-term exam | 8 | 2 | 40 |
| Other assessment methods | |||
| 1.Oral Examination | |||
| 2.Quiz | |||
| 3.Laboratory exam | |||
| 4.Presentation | |||
| 5.Report | |||
| 6.Workshop | |||
| 7.Performance Project | |||
| 8.Term Paper | |||
| 9.Project | |||
| final exam | 16 | 2 | 60 |
| Student Work Load | |||
| Type of Work | Weekly Hours | Number of Weeks | Work Load |
| Weekly Course Hours (Theoretical+Practice) | 2 | 14 | 28 |
| Outside Class | |||
| a) Reading | 2 | 6 | 12 |
| b) Search in internet/Library | 3 | 6 | 18 |
| c) Performance Project | 0 | ||
| d) Prepare a workshop/Presentation/Report | 2 | 5 | 10 |
| e) Term paper/Project | 0 | ||
| Oral Examination | 0 | ||
| Quiz | 0 | ||
| Laboratory exam | 0 | ||
| Own study for mid-term exam | 2 | 5 | 10 |
| mid-term exam | 1 | 1 | 1 |
| Own study for final exam | 2 | 5 | 10 |
| final exam | 1 | 1 | 1 |
| 0 | |||
| 0 | |||
| Total work load; | 90 | ||