Nevşehir Hacı Bektaş Veli University Course Catalogue
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| Year/Semester of Study | 1 / Fall Semester | ||||
| Level of Course | 2nd Cycle Degree Programme | ||||
| Type of Course | Optional | ||||
| Department | MONECULER BIOLOGY AND GENETICS | ||||
| Pre-requisities and Co-requisites | None | ||||
| Mode of Delivery | Face to Face | ||||
| Teaching Period | 14 Weeks | ||||
| Name of Lecturer | NAŞİT İĞCİ (nasitigci@nevsehir.edu.tr) | ||||
| Name of Lecturer(s) | |||||
| Language of Instruction | Turkish | ||||
| Work Placement(s) | None | ||||
| Objectives of the Course | |||||
| The aim of this course is to gain knowledge and skills about the basic principles of chromatography, chromatographic methods and its applications in molecular biology. | |||||
| Learning Outcomes | PO | MME | |
| The students who succeeded in this course: | |||
| LO-1 | can explain the basic concepts of chromatography. |
PO-1 Obtain basic knowledge of Molecular Biology PO-2 Have information about current topics in molecular biology and genetics PO-3 Ability to identify problems in Molecular Biology and genetics, to establish hypotheses for solving problems and to be able to solve hypothesis using various observational and experimental methods PO-6 Ability to independently carry out an activity that requires expertise in the field PO-8 Ability to participate in interdisciplinary studies in the fields of education, industry, agriculture, health, environment, energy and biotechnology PO-11 Explains the basic functions of scientific processes, critically discusses and solves problems using appropriate statistical and computational methods, chooses contemporary experimental and theoretical approaches that will ultimately lead to the solution of molecular biology and genetic research problems PO-13 Scientific developments contribute to research and development |
Examination |
| LO-2 | Can identify the chromatographic methods for different applications. |
PO-1 Obtain basic knowledge of Molecular Biology PO-2 Have information about current topics in molecular biology and genetics PO-3 Ability to identify problems in Molecular Biology and genetics, to establish hypotheses for solving problems and to be able to solve hypothesis using various observational and experimental methods PO-6 Ability to independently carry out an activity that requires expertise in the field PO-8 Ability to participate in interdisciplinary studies in the fields of education, industry, agriculture, health, environment, energy and biotechnology PO-11 Explains the basic functions of scientific processes, critically discusses and solves problems using appropriate statistical and computational methods, chooses contemporary experimental and theoretical approaches that will ultimately lead to the solution of molecular biology and genetic research problems PO-13 Scientific developments contribute to research and development |
Examination |
| LO-3 | can recognize modern devices used in chromatography. |
PO-1 Obtain basic knowledge of Molecular Biology PO-2 Have information about current topics in molecular biology and genetics PO-3 Ability to identify problems in Molecular Biology and genetics, to establish hypotheses for solving problems and to be able to solve hypothesis using various observational and experimental methods PO-6 Ability to independently carry out an activity that requires expertise in the field PO-8 Ability to participate in interdisciplinary studies in the fields of education, industry, agriculture, health, environment, energy and biotechnology PO-11 Explains the basic functions of scientific processes, critically discusses and solves problems using appropriate statistical and computational methods, chooses contemporary experimental and theoretical approaches that will ultimately lead to the solution of molecular biology and genetic research problems PO-13 Scientific developments contribute to research and development |
Examination |
| PO: Programme Outcomes MME:Method of measurement & Evaluation |
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| Course Contents | ||
| Historical development of chromatography, basic principles of chromatography, thin layer chromatography, gas chromatography, liquid chromatography, applications of chromatography in molecular biology | ||
| Weekly Course Content | ||
| Week | Subject | Learning Activities and Teaching Methods |
| 1 | Definition and history of chromatography. | Lecture, Discussion, Question and Answer, Literature Search |
| 2 | Theoretical basis of chromatography. | Lecture, Discussion, Question and Answer, Literature Search |
| 3 | Thin layer chromatography. | Lecture, Discussion, Question and Answer, Literature Search |
| 4 | Applications of thin layer chromatography. | Lecture, Discussion, Question and Answer, Literature Search |
| 5 | Gas chromatography. | Lecture, Discussion, Question and Answer, Literature Search |
| 6 | Applications of gas chromatography. | Lecture, Discussion, Question and Answer, Literature Search |
| 7 | Liquid-liquid chromatography. | Lecture, Discussion, Question and Answer, Literature Search |
| 8 | mid-term exam | |
| 9 | Adsorption chromatography. | Lecture, Discussion, Question and Answer, Literature Search |
| 10 | Liquid chromatography. | Lecture, Discussion, Question and Answer, Literature Search |
| 11 | Gel filtration chromatography, ion exchange and reversed phase chromatography. | Lecture, Discussion, Question and Answer, Literature Search |
| 12 | Affinity chromatography. | Lecture, Discussion, Question and Answer, Literature Search |
| 13 | Applications of liquid chromatography. | Lecture, Discussion, Question and Answer, Literature Search |
| 14 | Supercritical fluid chromatography. | Lecture, Discussion, Question and Answer, Literature Search |
| 15 | Modern instruments used for chromatography. | Lecture, Discussion, Question and Answer, Literature Search |
| 16 | final exam | |
| Recommend Course Book / Supplementary Book/Reading | ||
| 1 | Scott RPW. Techniques and Practice of Chromatography. Marcel Dekker, 1995. | |
| 2 | Gündüz, T. (2015). Kromatografi ve Elektroforez. Gazi Kitabevi. | |
| Required Course instruments and materials | ||
| Lecture notes, books, related articles, computer | ||
| Assessment Methods | |||
| Type of Assessment | Week | Hours | Weight(%) |
| mid-term exam | 8 | 1 | 40 |
| Other assessment methods | |||
| 1.Oral Examination | |||
| 2.Quiz | |||
| 3.Laboratory exam | |||
| 4.Presentation | |||
| 5.Report | |||
| 6.Workshop | |||
| 7.Performance Project | |||
| 8.Term Paper | |||
| 9.Project | |||
| final exam | 16 | 1 | 60 |
| Student Work Load | |||
| Type of Work | Weekly Hours | Number of Weeks | Work Load |
| Weekly Course Hours (Theoretical+Practice) | 3 | 14 | 42 |
| Outside Class | |||
| a) Reading | 4 | 14 | 56 |
| b) Search in internet/Library | 4 | 13 | 52 |
| c) Performance Project | 0 | ||
| d) Prepare a workshop/Presentation/Report | 0 | ||
| e) Term paper/Project | 0 | ||
| Oral Examination | 0 | ||
| Quiz | 0 | ||
| Laboratory exam | 0 | ||
| Own study for mid-term exam | 2 | 7 | 14 |
| mid-term exam | 1 | 1 | 1 |
| Own study for final exam | 2 | 7 | 14 |
| final exam | 1 | 1 | 1 |
| 0 | |||
| 0 | |||
| Total work load; | 180 | ||