Nevşehir Hacı Bektaş Veli University Course Catalogue
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| Year/Semester of Study | 1 / Spring Semester | ||||
| Level of Course | 2nd Cycle Degree Programme | ||||
| Type of Course | Optional | ||||
| Department | MONECULER BIOLOGY AND GENETICS | ||||
| Pre-requisities and Co-requisites | None | ||||
| Mode of Delivery | Face to Face | ||||
| Teaching Period | 14 Weeks | ||||
| Name of Lecturer | NAŞİT İĞCİ (nasitigci@nevsehir.edu.tr) | ||||
| Name of Lecturer(s) | |||||
| Language of Instruction | Turkish | ||||
| Work Placement(s) | None | ||||
| Objectives of the Course | |||||
| The aim of this course is to provide knowledge about experimental approaches, basic strategies and research methods in proteomics studies. | |||||
| Learning Outcomes | PO | MME | |
| The students who succeeded in this course: | |||
| LO-1 | can give information about the applications of proteomics. |
PO-2 Have information about current topics in molecular biology and genetics PO-3 Ability to identify problems in Molecular Biology and genetics, to establish hypotheses for solving problems and to be able to solve hypothesis using various observational and experimental methods PO-5 Ability to design a research project in the field of molecular biology and genetics and to evaluate and present the results related to it PO-6 Ability to independently carry out an activity that requires expertise in the field PO-7 Investigating the phenotypic effects of genes and acquiring the ability to use this effect in animal and plant breeding areas PO-8 Ability to participate in interdisciplinary studies in the fields of education, industry, agriculture, health, environment, energy and biotechnology PO-9 Recognizes and explains the molecular biology and genetics of the problems of other disciplines PO-10 Read and critically discuss the world-wide literature on molecular biology and molecular genetics PO-11 Explains the basic functions of scientific processes, critically discusses and solves problems using appropriate statistical and computational methods, chooses contemporary experimental and theoretical approaches that will ultimately lead to the solution of molecular biology and genetic research problems PO-12 To be able to transfer current developments and own studies in the field with quantitative and qualitative data to the groups in the field and outside the field in a systematic manner in written, oral and visual form PO-13 Scientific developments contribute to research and development |
Examination |
| LO-2 | learn and can apply current analyes in proteomics. |
PO-1 Obtain basic knowledge of Molecular Biology PO-2 Have information about current topics in molecular biology and genetics PO-3 Ability to identify problems in Molecular Biology and genetics, to establish hypotheses for solving problems and to be able to solve hypothesis using various observational and experimental methods PO-5 Ability to design a research project in the field of molecular biology and genetics and to evaluate and present the results related to it PO-6 Ability to independently carry out an activity that requires expertise in the field PO-7 Investigating the phenotypic effects of genes and acquiring the ability to use this effect in animal and plant breeding areas PO-8 Ability to participate in interdisciplinary studies in the fields of education, industry, agriculture, health, environment, energy and biotechnology PO-9 Recognizes and explains the molecular biology and genetics of the problems of other disciplines PO-10 Read and critically discuss the world-wide literature on molecular biology and molecular genetics PO-11 Explains the basic functions of scientific processes, critically discusses and solves problems using appropriate statistical and computational methods, chooses contemporary experimental and theoretical approaches that will ultimately lead to the solution of molecular biology and genetic research problems PO-12 To be able to transfer current developments and own studies in the field with quantitative and qualitative data to the groups in the field and outside the field in a systematic manner in written, oral and visual form PO-13 Scientific developments contribute to research and development |
Examination |
| PO: Programme Outcomes MME:Method of measurement & Evaluation |
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| Course Contents | ||
| Basic proteomics techniques, protein isolation, gel-based protein separation, chromatography-based protein separation, use of mass spectrometers in proteomics, protein identification with mass spectrometers, bioinformatics in proteomics. | ||
| Weekly Course Content | ||
| Week | Subject | Learning Activities and Teaching Methods |
| 1 | Basic structure and functions of proteins | Lecture, Discussion, Question Answer, Literature Search |
| 2 | Introduction to proteomics and its applications | Lecture, Discussion, Question Answer, Literature Search |
| 3 | Basic strategies in proteomics (top-down, bottom-up) | Lecture, Discussion, Question Answer, Literature Search |
| 4 | Protein isolation from various samples | Lecture, Discussion, Question Answer, Literature Search |
| 5 | Gel-based proteomic analyses | Lecture, Discussion, Question Answer, Literature Search |
| 6 | Protein separation by liquid chromatography | Lecture, Discussion, Question Answer, Literature Search |
| 7 | The use of mass spectrometry in proteomics, characteristics of different mass spectrometry systems | Lecture, Discussion, Question Answer, Literature Search |
| 8 | mid-term exam | |
| 9 | Experimental methods for mass spectrometry-based proteomci analyses | Lecture, Discussion, Question Answer, Literature Search |
| 10 | Interpretation and quality control of the results of a mass spectrometer | Lecture, Discussion, Question Answer, Literature Search |
| 11 | Protein identification by bioinformatic tools using mass spectrometry data | Lecture, Discussion, Question Answer, Literature Search |
| 12 | Antibody-based proteomic analyses | Lecture, Discussion, Question Answer, Literature Search |
| 13 | Determination of protein concentration | Lecture, Discussion, Question Answer, Literature Search |
| 14 | Laboratory exercise: SDS-PAGE | Lecture, Discussion, Question Answer, Literature Search |
| 15 | Bioinformatics exercise | Lecture, Discussion, Question Answer, Literature Search |
| 16 | final exam | |
| Recommend Course Book / Supplementary Book/Reading | ||
| 1 | Özel Demiralp F, İğci N, Peker S, Ayhan B. 2014. Temel Proteomik Stratejiler. Ankara Üniversitesi Yayınevi, Ankara. | |
| 2 | Hamdan M, Righetti PG. 2005. Proteomics Today. John Wiley & Sons, New Jersey. | |
| Required Course instruments and materials | ||
| Lecture notes, books, related articles, computer | ||
| Assessment Methods | |||
| Type of Assessment | Week | Hours | Weight(%) |
| mid-term exam | 8 | 1 | 40 |
| Other assessment methods | |||
| 1.Oral Examination | |||
| 2.Quiz | |||
| 3.Laboratory exam | |||
| 4.Presentation | |||
| 5.Report | |||
| 6.Workshop | |||
| 7.Performance Project | |||
| 8.Term Paper | |||
| 9.Project | |||
| final exam | 16 | 1 | 60 |
| Student Work Load | |||
| Type of Work | Weekly Hours | Number of Weeks | Work Load |
| Weekly Course Hours (Theoretical+Practice) | 3 | 14 | 42 |
| Outside Class | |||
| a) Reading | 4 | 14 | 56 |
| b) Search in internet/Library | 4 | 13 | 52 |
| c) Performance Project | 0 | ||
| d) Prepare a workshop/Presentation/Report | 0 | ||
| e) Term paper/Project | 0 | ||
| Oral Examination | 0 | ||
| Quiz | 0 | ||
| Laboratory exam | 0 | ||
| Own study for mid-term exam | 2 | 7 | 14 |
| mid-term exam | 1 | 1 | 1 |
| Own study for final exam | 2 | 7 | 14 |
| final exam | 1 | 1 | 1 |
| 0 | |||
| 0 | |||
| Total work load; | 180 | ||