Nevşehir Hacı Bektaş Veli University Course Catalogue
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| Year/Semester of Study | 1 / Spring Semester | ||||
| Level of Course | 2nd Cycle Degree Programme | ||||
| Type of Course | Optional | ||||
| Department | MONECULER BIOLOGY AND GENETICS | ||||
| Pre-requisities and Co-requisites | None | ||||
| Mode of Delivery | Face to Face | ||||
| Teaching Period | 14 Weeks | ||||
| Name of Lecturer | ÜMİT KUMBIÇAK (umitkumbicak@nevsehir.edu.tr) | ||||
| Name of Lecturer(s) | |||||
| Language of Instruction | Turkish | ||||
| Work Placement(s) | None | ||||
| Objectives of the Course | |||||
| The aim of this course is to gain knowledge and skills that can be used during cell culture studies. | |||||
| Learning Outcomes | PO | MME | |
| The students who succeeded in this course: | |||
| LO-1 | can explain the basic principles of cell culture studies. |
PO-1 Obtain basic knowledge of Molecular Biology PO-2 Have information about current topics in molecular biology and genetics PO-3 Ability to identify problems in Molecular Biology and genetics, to establish hypotheses for solving problems and to be able to solve hypothesis using various observational and experimental methods PO-4 Being ethical and responsible individuals who are sensitive to social and universal values in the field of Molecular Biology and genetics, who look after the priority areas of the country, research and produce and contribute to these issues PO-5 Ability to design a research project in the field of molecular biology and genetics and to evaluate and present the results related to it PO-6 Ability to independently carry out an activity that requires expertise in the field PO-7 Investigating the phenotypic effects of genes and acquiring the ability to use this effect in animal and plant breeding areas PO-8 Ability to participate in interdisciplinary studies in the fields of education, industry, agriculture, health, environment, energy and biotechnology PO-9 Recognizes and explains the molecular biology and genetics of the problems of other disciplines PO-10 Read and critically discuss the world-wide literature on molecular biology and molecular genetics |
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| LO-2 | can apply cell culture techniques. |
PO-1 Obtain basic knowledge of Molecular Biology PO-2 Have information about current topics in molecular biology and genetics PO-3 Ability to identify problems in Molecular Biology and genetics, to establish hypotheses for solving problems and to be able to solve hypothesis using various observational and experimental methods PO-4 Being ethical and responsible individuals who are sensitive to social and universal values in the field of Molecular Biology and genetics, who look after the priority areas of the country, research and produce and contribute to these issues PO-5 Ability to design a research project in the field of molecular biology and genetics and to evaluate and present the results related to it PO-6 Ability to independently carry out an activity that requires expertise in the field PO-7 Investigating the phenotypic effects of genes and acquiring the ability to use this effect in animal and plant breeding areas PO-8 Ability to participate in interdisciplinary studies in the fields of education, industry, agriculture, health, environment, energy and biotechnology PO-9 Recognizes and explains the molecular biology and genetics of the problems of other disciplines PO-10 Read and critically discuss the world-wide literature on molecular biology and molecular genetics |
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| LO-3 | can interpret results obtained from cell culture studies |
PO-1 Obtain basic knowledge of Molecular Biology PO-2 Have information about current topics in molecular biology and genetics PO-3 Ability to identify problems in Molecular Biology and genetics, to establish hypotheses for solving problems and to be able to solve hypothesis using various observational and experimental methods PO-4 Being ethical and responsible individuals who are sensitive to social and universal values in the field of Molecular Biology and genetics, who look after the priority areas of the country, research and produce and contribute to these issues PO-5 Ability to design a research project in the field of molecular biology and genetics and to evaluate and present the results related to it PO-6 Ability to independently carry out an activity that requires expertise in the field PO-7 Investigating the phenotypic effects of genes and acquiring the ability to use this effect in animal and plant breeding areas PO-8 Ability to participate in interdisciplinary studies in the fields of education, industry, agriculture, health, environment, energy and biotechnology PO-9 Recognizes and explains the molecular biology and genetics of the problems of other disciplines PO-10 Read and critically discuss the world-wide literature on molecular biology and molecular genetics PO-11 Explains the basic functions of scientific processes, critically discusses and solves problems using appropriate statistical and computational methods, chooses contemporary experimental and theoretical approaches that will ultimately lead to the solution of molecular biology and genetic research problems PO-12 To be able to transfer current developments and own studies in the field with quantitative and qualitative data to the groups in the field and outside the field in a systematic manner in written, oral and visual form PO-13 Scientific developments contribute to research and development |
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| PO: Programme Outcomes MME:Method of measurement & Evaluation |
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| Course Contents | ||
| Basic principles of cell culture, design of cell culture laboratory, special devices and materials used in cell culture laboratory, contamination, sterilization techniques, culture media and solutions, cell counting and analysis, methods for determining cell viability and proliferation, methods of determining cell death, tissue engineering | ||
| Weekly Course Content | ||
| Week | Subject | Learning Activities and Teaching Methods |
| 1 | The purpose and importance of cell culture applications | Explanation Method |
| 2 | Classification of the cell lines | Explanation Method |
| 3 | Tools and equipment used in cell culture laboratory | Explanation Method |
| 4 | Sterilization in cell culture | Explanation Method |
| 5 | Types of cell culture media and preparation | Explanation Method |
| 6 | Passaging methods in cell culture | Explanation Method |
| 7 | Freezing and Thawing Cells | Explanation Method |
| 8 | mid-term exam | |
| 9 | Sterility and microbial contamination tests | Explanation Method |
| 10 | Cell count and analysis | Explanation Method |
| 11 | Methods for determining cell viability | Explanation Method |
| 12 | Methods to determine cell proliferation | Explanation Method |
| 13 | Methods for determining cell death pathways | Explanation Method |
| 14 | Stem cell culture | Explanation Method |
| 15 | Tissue engineering | Explanation Method |
| 16 | final exam | |
| Recommend Course Book / Supplementary Book/Reading | ||
| 1 | Basic Cell Culture Protocols, Cheryl D. Helgason, Cindy L. Miller | |
| Required Course instruments and materials | ||
| Course book, course presentations | ||
| Assessment Methods | |||
| Type of Assessment | Week | Hours | Weight(%) |
| mid-term exam | 8 | 1 | 40 |
| Other assessment methods | |||
| 1.Oral Examination | |||
| 2.Quiz | |||
| 3.Laboratory exam | |||
| 4.Presentation | |||
| 5.Report | |||
| 6.Workshop | |||
| 7.Performance Project | |||
| 8.Term Paper | |||
| 9.Project | |||
| final exam | 16 | 2 | 60 |
| Student Work Load | |||
| Type of Work | Weekly Hours | Number of Weeks | Work Load |
| Weekly Course Hours (Theoretical+Practice) | 3 | 14 | 42 |
| Outside Class | |||
| a) Reading | 3 | 7 | 21 |
| b) Search in internet/Library | 4 | 8 | 32 |
| c) Performance Project | 4 | 7 | 28 |
| d) Prepare a workshop/Presentation/Report | 3 | 6 | 18 |
| e) Term paper/Project | 0 | ||
| Oral Examination | 0 | ||
| Quiz | 0 | ||
| Laboratory exam | 0 | ||
| Own study for mid-term exam | 3 | 6 | 18 |
| mid-term exam | 1 | 1 | 1 |
| Own study for final exam | 3 | 6 | 18 |
| final exam | 2 | 1 | 2 |
| 0 | |||
| 0 | |||
| Total work load; | 180 | ||