Nevşehir Hacı Bektaş Veli University Course Catalogue
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| Year/Semester of Study | 3 / Spring Semester | ||||
| Level of Course | 3rd Cycle Degree Programme | ||||
| Type of Course | Compulsory | ||||
| Department | MOLECULAR BIOLOGY AND GENETICS | ||||
| Pre-requisities and Co-requisites | None | ||||
| Mode of Delivery | Face to Face | ||||
| Teaching Period | 14 Weeks | ||||
| Name of Lecturer | ENVER ERSOY ANDEDEN (enverandeden@nevsehir.edu.tr) | ||||
| Name of Lecturer(s) | |||||
| Language of Instruction | Turkish | ||||
| Work Placement(s) | None | ||||
| Objectives of the Course | |||||
| Aim of the course is to provide the students with basic experience in a wide variety of biochemical research techniques, and perform chemical and enzymatic analysis using calorimetric, electrophoretic, spectrophotometric and chromatographic techniques. | |||||
| Learning Outcomes | PO | MME | |
| The students who succeeded in this course: | |||
| LO-1 | can characterize the purified enzymes. |
PO-1 Obtain basic knowledge of Biology PO-7 Work effectively in multi-disciplinary research teams PO-8 Develop an awareness of continuous learning in relation with modern technology |
Laboratory Exam |
| LO-2 | can apply gel filtration chromatography, and 3D protein analysis techniques for different protein labours. |
PO-1 Obtain basic knowledge of Biology PO-2 Develop an understanding of matter and energy in organisms PO-3 Identify structure-function relationships in cells and organisms PO-7 Work effectively in multi-disciplinary research teams PO-8 Develop an awareness of continuous learning in relation with modern technology PO-9 Find out new methods to improve his/her knowledge |
Laboratory Exam |
| LO-3 | can apply different methods for enzyme kinetics works. |
PO-7 Work effectively in multi-disciplinary research teams PO-8 Develop an awareness of continuous learning in relation with modern technology PO-9 Find out new methods to improve his/her knowledge PO-11 Apply biological concepts to personal, social, economical, technological and ethical issues PO-12 Demonstrate professional and ethical responsibility PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics PO-14 Have knowledge about the main research interests of the molecular biology and genetics PO-15 Have contributed to scientific advances with research and development abilities PO-17 Survey, analyze and interpret the experimental results and to reach a scientific result using the available scientific data. |
Laboratory Exam |
| LO-4 | can perform determination of several biomolecules. |
PO-3 Identify structure-function relationships in cells and organisms PO-7 Work effectively in multi-disciplinary research teams PO-8 Develop an awareness of continuous learning in relation with modern technology PO-9 Find out new methods to improve his/her knowledge PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics PO-14 Have knowledge about the main research interests of the molecular biology and genetics PO-15 Have contributed to scientific advances with research and development abilities |
Laboratory Exam |
| PO: Programme Outcomes MME:Method of measurement & Evaluation |
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| Course Contents | ||
| Characterization of LDH with Gel filtration chromatography and 3D Protein analysis, Enzyme kinetics; determination of Km, inhibition kinetics, Determination of total blood protein, lipid amount, Qualitative determination of carbohydrates and aminoacids, Tests for reductive sugars, Analysis of plant pigments, Determination of antioxidant activity, Determination of Vitamin C (Ascorbic acid) | ||
| Weekly Course Content | ||
| Week | Subject | Learning Activities and Teaching Methods |
| 1 | Characterization of LDH,Gel filtration chromatography I | Explanation, Experimentation |
| 2 | Characterization of LDH,Gel filtration chromatography II | Explanation, Experimentation |
| 3 | Characterization of LDH, 3D Protein analysis III | Explanation, Experimentation |
| 4 | Enzyme kinetics; determination of Km value | Explanation, Experimentation |
| 5 | Enzyme kinetics; inhibition kinetics | Explanation, Experimentation |
| 6 | Enzyme kinetics; inhibition kinetics | Explanation, Experimentation |
| 7 | Determination of total blood protein amount | Explanation, Experimentation |
| 8 | mid-term exam | |
| 9 | Determination of total blood lipid amount | Explanation, Experimentation |
| 10 | Qualitative determination of carbohydrates | Explanation, Experimentation |
| 11 | Qualitative determination of amino acids | Explanation, Experimentation |
| 12 | Tests for reductive sugars | Explanation, Experimentation |
| 13 | Analysis of plant pigments | Explanation, Experimentation |
| 14 | Determination of antioxidant activity | Explanation, Experimentation |
| 15 | Determination of Vitamin C (Ascorbic acid) | Explanation, Experimentation |
| 16 | final exam | |
| Recommend Course Book / Supplementary Book/Reading | ||
| 1 | Nelson DL ve Cox MM, 2008. Lehninger Principles of Biochemistry (5. basım), WH Freeman | |
| 2 | Laboratuvar Föyü | |
| Required Course instruments and materials | ||
| Laboratory catalogs, Lecture Notes, basic laboratory material | ||
| Assessment Methods | |||
| Type of Assessment | Week | Hours | Weight(%) |
| mid-term exam | 8 | 2 | 40 |
| Other assessment methods | |||
| 1.Oral Examination | |||
| 2.Quiz | |||
| 3.Laboratory exam | |||
| 4.Presentation | |||
| 5.Report | |||
| 6.Workshop | |||
| 7.Performance Project | |||
| 8.Term Paper | |||
| 9.Project | |||
| final exam | 16 | 2 | 60 |
| Student Work Load | |||
| Type of Work | Weekly Hours | Number of Weeks | Work Load |
| Weekly Course Hours (Theoretical+Practice) | 2 | 14 | 28 |
| Outside Class | |||
| a) Reading | 1 | 14 | 14 |
| b) Search in internet/Library | 1 | 14 | 14 |
| c) Performance Project | 0 | ||
| d) Prepare a workshop/Presentation/Report | 0 | ||
| e) Term paper/Project | 0 | ||
| Oral Examination | 0 | ||
| Quiz | 0 | ||
| Laboratory exam | 0 | ||
| Own study for mid-term exam | 2 | 7 | 14 |
| mid-term exam | 2 | 1 | 2 |
| Own study for final exam | 2 | 8 | 16 |
| final exam | 2 | 1 | 2 |
| 0 | |||
| 0 | |||
| Total work load; | 90 | ||