Nevşehir Hacı Bektaş Veli University Course Catalogue
|
|||||
| Year/Semester of Study | 3 / Spring Semester | ||||
| Level of Course | 1st Cycle Degree Programme | ||||
| Type of Course | Optional | ||||
| Department | MOLECULAR BIOLOGY AND GENETICS | ||||
| Pre-requisities and Co-requisites | None | ||||
| Mode of Delivery | Face to Face | ||||
| Teaching Period | 14 Weeks | ||||
| Name of Lecturer | ÜMİT KUMBIÇAK (umitkumbicak@nevsehir.edu.tr) | ||||
| Name of Lecturer(s) | |||||
| Language of Instruction | Turkish | ||||
| Work Placement(s) | None | ||||
| Objectives of the Course | |||||
| The aim of this course is to gain knowledge and skills about plant and animal cell and tissue culture applications. | |||||
| Learning Outcomes | PO | MME | |
| The students who succeeded in this course: | |||
| LO-1 | Can explain the basic concepts of tissue culture. |
PO-1 Obtain basic knowledge of Biology PO-3 Identify structure-function relationships in cells and organisms PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics PO-15 Have contributed to scientific advances with research and development abilities |
Examination |
| LO-2 | Can known the tissue culture techniques. |
PO-1 Obtain basic knowledge of Biology PO-8 Develop an awareness of continuous learning in relation with modern technology PO-9 Find out new methods to improve his/her knowledge PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics PO-14 Have knowledge about the main research interests of the molecular biology and genetics |
Examination |
| LO-3 | Can explain the areas of tissue culture techniques. |
PO-1 Obtain basic knowledge of Biology PO-2 Develop an understanding of matter and energy in organisms PO-3 Identify structure-function relationships in cells and organisms PO-4 Define the relationship among life forms and their environments and ecosystems PO-10 Demonstrate sufficiency in English to follow literature, present technical projects and write articles PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics PO-14 Have knowledge about the main research interests of the molecular biology and genetics |
Examination |
| PO: Programme Outcomes MME:Method of measurement & Evaluation |
|||
| Course Contents | ||
| Importance of plant and animal cell and tissue culture studies, tools and equipment used in cell and tissue culture laboratories, sources of contamination and sterilization methods, stages of plant tissue culture, types of animal cell culture according to their sources and growth patterns, preparation of medium, feeding and passaging of cells, cell freezing and dissolving processes, cell counting and analysis, cell viability determination methods, stem cell definition, types and application areas | ||
| Weekly Course Content | ||
| Week | Subject | Learning Activities and Teaching Methods |
| 1 | Introduction to cell and tissue culture: Plant tissue cultures, Animal cell and tissue cultures | Explaining technique |
| 2 | Importance, aim, advantages and disadvantages of plant tissue culture studies | Explaining technique |
| 3 | Tools and equipment used in plant cell and tissue culture laboratories, Contamination sources and sterilization methods in plant tissue culture | Explaining technique |
| 4 | Plant tissue culture stages, Plant tissue culture media and plant growth regulators | Explaining technique |
| 5 | Application areas of plant tissue cultures, Somaclonal variation, protoplast fusion, gene transfer | Explaining technique |
| 6 | Micropropagation, synthetic seed production, embryo culture, haploid plant production, in vitro selection | Explaining technique |
| 7 | production of secondary metabolites, chimeras, in vitro fertilization, in vitro germplasm storage, meristem culture | Explaining technique |
| 8 | mid-term exam | |
| 9 | Importance, advantages and disadvantages of animal cell and tissue culture applications | Explaining technique |
| 10 | Equipment used in animal cell culture laboratory, Contamination sources and sterilization methods in animal cell culture | Explaining technique |
| 11 | Varieties of animal cell cultures according to their sources and growth patterns | Explaining technique |
| 12 | Cell culture medium preparation, feeding cells and passaging methods | Explaining technique |
| 13 | Freezing and Thawing Cells | Explaining technique |
| 14 | Cell count and analysis Methods for determining cell viability | Explaining technique |
| 15 | Stem cell definition, types and application areas | Explaining technique |
| 16 | final exam | |
| Recommend Course Book / Supplementary Book/Reading | ||
| 1 | Kocaçalışkan, İ. 2017. "Doku ve Hücre Kültürü Teknikleri" | |
| Required Course instruments and materials | ||
| Powerpoint Presantations | ||
| Assessment Methods | |||
| Type of Assessment | Week | Hours | Weight(%) |
| mid-term exam | 8 | 2 | 40 |
| Other assessment methods | |||
| 1.Oral Examination | |||
| 2.Quiz | |||
| 3.Laboratory exam | |||
| 4.Presentation | |||
| 5.Report | |||
| 6.Workshop | |||
| 7.Performance Project | |||
| 8.Term Paper | |||
| 9.Project | |||
| final exam | 16 | 2 | 60 |
| Student Work Load | |||
| Type of Work | Weekly Hours | Number of Weeks | Work Load |
| Weekly Course Hours (Theoretical+Practice) | 2 | 14 | 28 |
| Outside Class | |||
| a) Reading | 2 | 6 | 12 |
| b) Search in internet/Library | 3 | 6 | 18 |
| c) Performance Project | 0 | ||
| d) Prepare a workshop/Presentation/Report | 2 | 5 | 10 |
| e) Term paper/Project | 0 | ||
| Oral Examination | 0 | ||
| Quiz | 0 | ||
| Laboratory exam | 0 | ||
| Own study for mid-term exam | 2 | 5 | 10 |
| mid-term exam | 1 | 1 | 1 |
| Own study for final exam | 2 | 5 | 10 |
| final exam | 1 | 1 | 1 |
| 0 | |||
| 0 | |||
| Total work load; | 90 | ||