Nevşehir Hacı Bektaş Veli University Course Catalogue
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| Year/Semester of Study | 2 / Fall Semester | ||||
| Level of Course | 3rd Cycle Degree Programme | ||||
| Type of Course | Compulsory | ||||
| Department | MOLECULAR BIOLOGY AND GENETICS | ||||
| Pre-requisities and Co-requisites | None | ||||
| Mode of Delivery | Face to Face | ||||
| Teaching Period | 14 Weeks | ||||
| Name of Lecturer | ENVER ERSOY ANDEDEN (enverandeden@nevsehir.edu.tr) | ||||
| Name of Lecturer(s) | |||||
| Language of Instruction | Turkish | ||||
| Work Placement(s) | None | ||||
| Objectives of the Course | |||||
| Aim of the course is to teach basic techniques used in molecular biology and recombinant DNA methods with applications. | |||||
| Learning Outcomes | PO | MME | |
| The students who succeeded in this course: | |||
| LO-1 | can perform basic recombinant DNA techniques. |
PO-7 Work effectively in multi-disciplinary research teams PO-8 Develop an awareness of continuous learning in relation with modern technology |
Examination |
| LO-2 | can isolate and purify DNA and RNA have a view about PCR and perform PCR . |
PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics PO-14 Have knowledge about the main research interests of the molecular biology and genetics PO-16 Explain the scientific findings clearly and efficiently both in written and verbal communications. |
Examination |
| LO-3 | can perform molecular clonning experiments. |
PO-5 Explain transmission of genetic background in organisms and populations PO-9 Find out new methods to improve his/her knowledge PO-15 Have contributed to scientific advances with research and development abilities PO-17 Survey, analyze and interpret the experimental results and to reach a scientific result using the available scientific data. |
Examination |
| PO: Programme Outcomes MME:Method of measurement & Evaluation |
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| Course Contents | ||
| DNA and RNA isolation and determination of quality and quantity via spechtrophotometric analysis Isolation DNA, and agarose gel electrophoresis, PCR amplification of DNA, Restriction enzyme digestion of DNA, Purification of DNA from agarose gel, Molecular cloning, Transformation, Blue-white colony screening, Southern Blotting, cDNA library, Real time and gradient PCR analysis | ||
| Weekly Course Content | ||
| Week | Subject | Learning Activities and Teaching Methods |
| 1 | Introduction, Molecular Biology Lab rules, techniques for writing lab notebook and report | Explanation, Experimentation |
| 2 | DNA isolation from several samples and determination of quality and quantity via spechtrophotometric analysis | Explanation, Experimentation |
| 3 | Explanation of areas of gene cloning, Isolation of genomic DNA from bacteria, and agarose gel electrophoresis | Explanation, Experimentation |
| 4 | PCR amplification of DNA | Explanation, Experimentation |
| 5 | Restriction enzyme digestion of DNA | Explanation, Experimentation |
| 6 | Purification of DNA from agarose gel with several methods | Explanation, Experimentation |
| 7 | Molecular cloning | Explanation, Experimentation |
| 8 | mid-term exam | |
| 9 | Transformation of E.coli by electroporation | Explanation, Experimentation |
| 10 | Selection of organisms containing vector sequences, Screening for clones with desired DNA inserts | Explanation, Experimentation |
| 11 | Southern Blotting | Explanation, Experimentation |
| 12 | RNA isolation from several samples and determination of quality and quantity via spechtrophotometric analysis | Explanation, Experimentation |
| 13 | RNA isolation and making cDNA library | Explanation, Experimentation |
| 14 | Real time PCR analysis | Explanation, Experimentation |
| 15 | Gradient PCR analysis | Explanation, Experimentation |
| 16 | final exam | |
| Recommend Course Book / Supplementary Book/Reading | ||
| 1 | Watson J. ve diğerleri, 2008. Molecular Biology of the Gene, (7. baskı). San Francisco Pearson yayınları | |
| 2 | Davis LG, Dibner MD, Battey JF. 1986. Basic Methods in Molecular Biology. Elsevier | |
| Required Course instruments and materials | ||
| Laboratory Catalog, Lecture Notes, basic laboratory material | ||
| Assessment Methods | |||
| Type of Assessment | Week | Hours | Weight(%) |
| mid-term exam | 8 | 2 | 40 |
| Other assessment methods | |||
| 1.Oral Examination | |||
| 2.Quiz | |||
| 3.Laboratory exam | |||
| 4.Presentation | |||
| 5.Report | |||
| 6.Workshop | |||
| 7.Performance Project | |||
| 8.Term Paper | |||
| 9.Project | |||
| final exam | 16 | 2 | 60 |
| Student Work Load | |||
| Type of Work | Weekly Hours | Number of Weeks | Work Load |
| Weekly Course Hours (Theoretical+Practice) | 4 | 14 | 56 |
| Outside Class | |||
| a) Reading | 1 | 14 | 14 |
| b) Search in internet/Library | 1 | 14 | 14 |
| c) Performance Project | 0 | ||
| d) Prepare a workshop/Presentation/Report | 0 | ||
| e) Term paper/Project | 0 | ||
| Oral Examination | 0 | ||
| Quiz | 0 | ||
| Laboratory exam | 0 | ||
| Own study for mid-term exam | 2 | 8 | 16 |
| mid-term exam | 2 | 1 | 2 |
| Own study for final exam | 2 | 8 | 16 |
| final exam | 2 | 1 | 2 |
| 0 | |||
| 0 | |||
| Total work load; | 120 | ||