Nevşehir Hacı Bektaş Veli University Course Catalogue
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| Year/Semester of Study | 4 / Fall Semester | ||||
| Level of Course | 1st Cycle Degree Programme | ||||
| Type of Course | Compulsory | ||||
| Department | MOLECULAR BIOLOGY AND GENETICS | ||||
| Pre-requisities and Co-requisites | None | ||||
| Mode of Delivery | Face to Face | ||||
| Teaching Period | 14 Weeks | ||||
| Name of Lecturer | NAŞİT İĞCİ (nasitigci@nevsehir.edu.tr) | ||||
| Name of Lecturer(s) | |||||
| Language of Instruction | Turkish | ||||
| Work Placement(s) | None | ||||
| Objectives of the Course | |||||
| Aim of the lecture is to give general information about extent and history of biotechnology and endustry of biotechnology, and teach basic methods and principles that are used in recombinant DNA technologies area. | |||||
| Learning Outcomes | PO | MME | |
| The students who succeeded in this course: | |||
| LO-1 | can explain the basic concepts of content and history of biotechnology and biotechnology endustry. |
PO-1 Obtain basic knowledge of Biology PO-8 Develop an awareness of continuous learning in relation with modern technology PO-9 Find out new methods to improve his/her knowledge PO-11 Apply biological concepts to personal, social, economical, technological and ethical issues |
Examination |
| LO-2 | can use the basic methods that are used in biotechnology. |
PO-8 Develop an awareness of continuous learning in relation with modern technology PO-9 Find out new methods to improve his/her knowledge PO-11 Apply biological concepts to personal, social, economical, technological and ethical issues |
Examination |
| LO-3 | Can known PCR types and molecular markers. |
PO-7 Work effectively in multi-disciplinary research teams PO-10 Demonstrate sufficiency in English to follow literature, present technical projects and write articles PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics PO-14 Have knowledge about the main research interests of the molecular biology and genetics PO-15 Have contributed to scientific advances with research and development abilities PO-16 Explain the scientific findings clearly and efficiently both in written and verbal communications. |
Examination |
| PO: Programme Outcomes MME:Method of measurement & Evaluation |
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| Course Contents | ||
| History of biotechnology, isolation, purification and determination of nucleic acids and genomic DNA, Chemical synthesis of DNA, Polymerase Chain Reactions and types, Molecular markers, Nucleic acid labeling methods, Genomic analysis, Gene silencing, gene mutations, DNA chips | ||
| Weekly Course Content | ||
| Week | Subject | Learning Activities and Teaching Methods |
| 1 | Definition and history of biotechnology | Explanation |
| 2 | Isolation of nucleic acids | Explanation |
| 3 | Restriction enzymes, Ligation | Explanation |
| 4 | Procaryotic genomic DNA and plasmid isolation | Explanation |
| 5 | Eucaryotic DNA isolation | Explanation |
| 6 | Purification of nucleis acids and determination of purification; spechtrophotometric method, electrophoretic method | Explanation |
| 7 | Chemical synthesis of DNA and Sequence analysis | Explanation |
| 8 | mid-term exam | |
| 9 | Polymerase Chain Reactions and types; Asyymetric PCR (Late-PCR), Nested PCR, Multiplex PCR, Competitive PCR, Real time PCR, RT-PCR | Explanation |
| 10 | Analysis of DNA polymorphism with moleculer methods (molecular markers) | Explanation |
| 11 | Molecular markers, Usage of molecular markers in genetic analysis and medicine | Explanation |
| 12 | Nucleic acid labeling methods, Nucleic acid hybridization methods | Explanation |
| 13 | Genomic analysis; Comparison of genomic sequences, determination of genetic difference according to sequences | Explanation |
| 14 | Gene silencing, gene mutations | Explanation |
| 15 | DNA chips | Explanation |
| 16 | final exam | |
| Recommend Course Book / Supplementary Book/Reading | ||
| 1 | John M. Walker, Ralph Rapley, 2000. Molecular Biology and Biotechnology 3. basım. Royal Society of Chemistry | |
| Required Course instruments and materials | ||
| John M. Walker, Ralph Rapley, 2000. Molecular Biology and Biotechnology 3rd edition. Royal Society of Chemistry | ||
| Assessment Methods | |||
| Type of Assessment | Week | Hours | Weight(%) |
| mid-term exam | 8 | 2 | 40 |
| Other assessment methods | |||
| 1.Oral Examination | |||
| 2.Quiz | |||
| 3.Laboratory exam | |||
| 4.Presentation | |||
| 5.Report | |||
| 6.Workshop | |||
| 7.Performance Project | |||
| 8.Term Paper | |||
| 9.Project | |||
| final exam | 16 | 2 | 60 |
| Student Work Load | |||
| Type of Work | Weekly Hours | Number of Weeks | Work Load |
| Weekly Course Hours (Theoretical+Practice) | 3 | 14 | 42 |
| Outside Class | |||
| a) Reading | 2 | 10 | 20 |
| b) Search in internet/Library | 2 | 13 | 26 |
| c) Performance Project | 0 | ||
| d) Prepare a workshop/Presentation/Report | 0 | ||
| e) Term paper/Project | 0 | ||
| Oral Examination | 0 | ||
| Quiz | 0 | ||
| Laboratory exam | 0 | ||
| Own study for mid-term exam | 2 | 7 | 14 |
| mid-term exam | 2 | 1 | 2 |
| Own study for final exam | 2 | 7 | 14 |
| final exam | 2 | 1 | 2 |
| 0 | |||
| 0 | |||
| Total work load; | 120 | ||