Nevşehir Hacı Bektaş Veli University Course Catalogue
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| Year/Semester of Study | 4 / Spring Semester | ||||
| Level of Course | 1st Cycle Degree Programme | ||||
| Type of Course | Compulsory | ||||
| Department | MOLECULAR BIOLOGY AND GENETICS | ||||
| Pre-requisities and Co-requisites | None | ||||
| Mode of Delivery | Face to Face | ||||
| Teaching Period | 14 Weeks | ||||
| Name of Lecturer | NAŞİT İĞCİ (nasitigci@nevsehir.edu.tr) | ||||
| Name of Lecturer(s) | |||||
| Language of Instruction | Turkish | ||||
| Work Placement(s) | None | ||||
| Objectives of the Course | |||||
| Aim of the lecture is to teach basic methods and principles that are used in recombinant DNA and protein technologies and give information about relation between modern biotechnology and molecular biology and their applications on other areas such as medicine. | |||||
| Learning Outcomes | PO | MME | |
| The students who succeeded in this course: | |||
| LO-1 | can explain the protein and enzyme engineering and the basic methods that are used in protein and enzyme engineering. |
PO-8 Develop an awareness of continuous learning in relation with modern technology PO-9 Find out new methods to improve his/her knowledge PO-11 Apply biological concepts to personal, social, economical, technological and ethical issues |
Examination |
| LO-2 | can give information about genomics and proteomics and give new ideas about those. |
PO-6 Develop an understanding of nature and history of scientific thought PO-8 Develop an awareness of continuous learning in relation with modern technology PO-9 Find out new methods to improve his/her knowledge PO-10 Demonstrate sufficiency in English to follow literature, present technical projects and write articles PO-11 Apply biological concepts to personal, social, economical, technological and ethical issues PO-12 Demonstrate professional and ethical responsibility |
Examination |
| LO-3 | can hold a view about genetically modified organisms and explain their intended use on agriculture, medicine and biotechnology. |
PO-8 Develop an awareness of continuous learning in relation with modern technology PO-9 Find out new methods to improve his/her knowledge PO-11 Apply biological concepts to personal, social, economical, technological and ethical issues PO-12 Demonstrate professional and ethical responsibility PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics PO-14 Have knowledge about the main research interests of the molecular biology and genetics |
Examination |
| PO: Programme Outcomes MME:Method of measurement & Evaluation |
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| Course Contents | ||
| Protein Isolation and purification, determination of protein concentration, Protein Electrophoresis, Gel staining techniques, hybridization techniques, Determination of amino acid concentration of proteins, Enzymatic activity and determination methods, Proteomic analysis, Protein and enzyme engineering technology, Genetically modified organisms and gene therapy | ||
| Weekly Course Content | ||
| Week | Subject | Learning Activities and Teaching Methods |
| 1 | Microscope types and usage purposes | Explanation |
| 2 | Introduction to molecular methods | Explanation |
| 3 | Physical molecular Methods | Explanation |
| 4 | Centrifuge and Chromatography | Explanation |
| 5 | Gel staining techniques | Explanation |
| 6 | Membrane transfer and identification methoda (Western Blotting), Other hybridization techniques | Explanation |
| 7 | Protein purification techniques, crystalization and purification control | Explanation |
| 8 | mid-term exam | |
| 9 | Determination of amino acid concentration of proteins | Explanation |
| 10 | Enzymatic activity and determination methods | Explanation |
| 11 | Determination of DNA-protein relation | Explanation |
| 12 | Proteomic analysis | Explanation |
| 13 | Protein engineering technology; Recombinant proteins and directed mutations | Explanation |
| 14 | Enzyme engineering and technology | Explanation |
| 15 | Genetically modified organisms and gene therapy | Explanation |
| 16 | final exam | |
| Recommend Course Book / Supplementary Book/Reading | ||
| 1 | Frederick M. Ausubel, Current Protocols in Molecular Biology, John Wiley & Sons Inc, 2003. | |
| 2 | Mary C. Phelan Current Protocols in Cell Biology, John Wiley & Sons, Inc, 1998 | |
| Required Course instruments and materials | ||
| Frederick M. Ausubel, Current Protocols in Molecular Biology, John Wiley & Sons Inc, 2003. | ||
| Assessment Methods | |||
| Type of Assessment | Week | Hours | Weight(%) |
| mid-term exam | 8 | 2 | 40 |
| Other assessment methods | |||
| 1.Oral Examination | |||
| 2.Quiz | |||
| 3.Laboratory exam | |||
| 4.Presentation | |||
| 5.Report | |||
| 6.Workshop | |||
| 7.Performance Project | |||
| 8.Term Paper | |||
| 9.Project | |||
| final exam | 16 | 2 | 60 |
| Student Work Load | |||
| Type of Work | Weekly Hours | Number of Weeks | Work Load |
| Weekly Course Hours (Theoretical+Practice) | 3 | 14 | 42 |
| Outside Class | |||
| a) Reading | 1 | 8 | 8 |
| b) Search in internet/Library | 1 | 8 | 8 |
| c) Performance Project | 0 | ||
| d) Prepare a workshop/Presentation/Report | 0 | ||
| e) Term paper/Project | 0 | ||
| Oral Examination | 0 | ||
| Quiz | 0 | ||
| Laboratory exam | 0 | ||
| Own study for mid-term exam | 2 | 7 | 14 |
| mid-term exam | 2 | 1 | 2 |
| Own study for final exam | 2 | 7 | 14 |
| final exam | 2 | 1 | 2 |
| 0 | |||
| 0 | |||
| Total work load; | 90 | ||