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Year/Semester of Study | 3 / Fall Semester | ||||
Level of Course | 1st Cycle Degree Programme | ||||
Type of Course | Optional | ||||
Department | MOLECULAR BIOLOGY AND GENETICS | ||||
Pre-requisities and Co-requisites | None | ||||
Mode of Delivery | Face to Face | ||||
Teaching Period | 14 Weeks | ||||
Name of Lecturer | ÜMİT KUMBIÇAK (umitkumbicak@nevsehir.edu.tr) | ||||
Name of Lecturer(s) | |||||
Language of Instruction | Turkish | ||||
Work Placement(s) | None | ||||
Objectives of the Course | |||||
To teach the areas of genetic engineering techniques, the products produced by genetic engineering, and the products obtained from genetically modified organisms. |
Learning Outcomes | PO | MME | |
The students who succeeded in this course: | |||
LO-1 | Can explain the applications and techniques of genetic engineering |
PO-1 Obtain basic knowledge of Biology PO-5 Explain transmission of genetic background in organisms and populations PO-8 Develop an awareness of continuous learning in relation with modern technology |
Examination Presentation |
LO-2 | Can explain the gene cloning steps and strategies |
PO-9 Find out new methods to improve his/her knowledge PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics PO-14 Have knowledge about the main research interests of the molecular biology and genetics |
Examination Presentation |
LO-3 | Can explain the methods of obtaining transgenic creatures |
PO-11 Apply biological concepts to personal, social, economical, technological and ethical issues PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics PO-14 Have knowledge about the main research interests of the molecular biology and genetics |
Examination Presentation |
PO: Programme Outcomes MME:Method of measurement & Evaluation |
Course Contents | ||
Genetic engineering areas, and strategies of cloning steps , the construction of genomic DNA and cDNA libraries , siRNA and miRNA technology, cloning organisms and transgenic organisms, cloning and purification of protein. | ||
Weekly Course Content | ||
Week | Subject | Learning Activities and Teaching Methods |
1 | Introduction to genetic engineering, definition of concepts and application areas of genetic engineering | Explaining, question and answer teaching method |
2 | Structure of the genome, replication, transcription and translation | Explaining, question and answer teaching method |
3 | Enzymes and molecular biological methods used in genetic engineering | Explaining, question and answer teaching method |
4 | Vectors used in genetic engineering and their properties | Explaining, question and answer teaching method |
5 | Host organism types and gene transfer methods | Explaining, question and answer teaching method |
6 | Gene cloning techniques, clone detection methods | Explaining, question and answer teaching method |
7 | Basic principles and usage areas of nucleic acid hybridization methods | Explaining, question and answer teaching method |
8 | mid-term exam | |
9 | DNA isolation, Genomic DNA and cDNA libraries | Explaining, question and answer teaching method |
10 | Genome analysis and human genome project | Explaining, question and answer teaching method |
11 | Recombinant DNA recovery and products obtained by Recombinant DNA technology (protein, vaccine and hormone production) | Explaining, question and answer teaching method |
12 | siRNA and miRNA technology and gene therapy applications and student presentations | Explaining, question and answer teaching method |
13 | Genetic applications in somatic cells. Protoplast isolation, fusion and somatic hybrids, reconstructed cells | Explaining, question and answer teaching method |
14 | Organism cloning, transgenic creatures | Explaining, question and answer teaching method |
15 | The impacts and future of recombinant DNA technology and genetic engineering and student presentations | Explaining, question and answer teaching method |
16 | final exam | |
Recommend Course Book / Supplementary Book/Reading | ||
1 | An Introduction to Genetic Engineering, DST. Nicholl, 3. Edition, Cambridge University Press, 2008. | |
Required Course instruments and materials | ||
Text Book |
Assessment Methods | |||
Type of Assessment | Week | Hours | Weight(%) |
mid-term exam | 8 | 2 | 40 |
Other assessment methods | |||
1.Oral Examination | |||
2.Quiz | |||
3.Laboratory exam | |||
4.Presentation | |||
5.Report | |||
6.Workshop | |||
7.Performance Project | |||
8.Term Paper | |||
9.Project | |||
final exam | 16 | 2 | 60 |
Student Work Load | |||
Type of Work | Weekly Hours | Number of Weeks | Work Load |
Weekly Course Hours (Theoretical+Practice) | 2 | 14 | 28 |
Outside Class | |||
a) Reading | 2 | 6 | 12 |
b) Search in internet/Library | 3 | 6 | 18 |
c) Performance Project | 0 | ||
d) Prepare a workshop/Presentation/Report | 2 | 5 | 10 |
e) Term paper/Project | 0 | ||
Oral Examination | 0 | ||
Quiz | 0 | ||
Laboratory exam | 0 | ||
Own study for mid-term exam | 2 | 5 | 10 |
mid-term exam | 1 | 1 | 1 |
Own study for final exam | 2 | 5 | 10 |
final exam | 1 | 1 | 1 |
0 | |||
0 | |||
Total work load; | 90 |