Nevşehir Hacı Bektaş Veli University Course Catalogue

Information Of Programmes

FACULTY OF LETTERS & SCIENCE / MBG 319 - MOLECULAR BIOLOGY AND GENETICS

Code: MBG 319 Course Title: GENETIC ENGINEERING Theoretical+Practice: 2+0 ECTS: 3
Year/Semester of Study 3 / Fall Semester
Level of Course 1st Cycle Degree Programme
Type of Course Optional
Department MOLECULAR BIOLOGY AND GENETICS
Pre-requisities and Co-requisites None
Mode of Delivery Face to Face
Teaching Period 14 Weeks
Name of Lecturer ÜMİT KUMBIÇAK (umitkumbicak@nevsehir.edu.tr)
Name of Lecturer(s)
Language of Instruction Turkish
Work Placement(s) None
Objectives of the Course
To teach the areas of genetic engineering techniques, the products produced by genetic engineering, and the products obtained from genetically modified organisms.

Learning Outcomes PO MME
The students who succeeded in this course:
LO-1 Can explain the applications and techniques of genetic engineering PO-1 Obtain basic knowledge of Biology
PO-5 Explain transmission of genetic background in organisms and populations
PO-8 Develop an awareness of continuous learning in relation with modern technology
Examination
Presentation
LO-2 Can explain the gene cloning steps and strategies PO-9 Find out new methods to improve his/her knowledge
PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics
PO-14 Have knowledge about the main research interests of the molecular biology and genetics
Examination
Presentation
LO-3 Can explain the methods of obtaining transgenic creatures PO-11 Apply biological concepts to personal, social, economical, technological and ethical issues
PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics
PO-14 Have knowledge about the main research interests of the molecular biology and genetics
Examination
Presentation
PO: Programme Outcomes
MME:Method of measurement & Evaluation

Course Contents
Genetic engineering areas, and strategies of cloning steps , the construction of genomic DNA and cDNA libraries , siRNA and miRNA technology, cloning organisms and transgenic organisms, cloning and purification of protein.
Weekly Course Content
Week Subject Learning Activities and Teaching Methods
1 Introduction to genetic engineering, definition of concepts and application areas of genetic engineering Explaining, question and answer teaching method
2 Structure of the genome, replication, transcription and translation Explaining, question and answer teaching method
3 Enzymes and molecular biological methods used in genetic engineering Explaining, question and answer teaching method
4 Vectors used in genetic engineering and their properties Explaining, question and answer teaching method
5 Host organism types and gene transfer methods Explaining, question and answer teaching method
6 Gene cloning techniques, clone detection methods Explaining, question and answer teaching method
7 Basic principles and usage areas of nucleic acid hybridization methods Explaining, question and answer teaching method
8 mid-term exam
9 DNA isolation, Genomic DNA and cDNA libraries Explaining, question and answer teaching method
10 Genome analysis and human genome project Explaining, question and answer teaching method
11 Recombinant DNA recovery and products obtained by Recombinant DNA technology (protein, vaccine and hormone production) Explaining, question and answer teaching method
12 siRNA and miRNA technology and gene therapy applications and student presentations Explaining, question and answer teaching method
13 Genetic applications in somatic cells. Protoplast isolation, fusion and somatic hybrids, reconstructed cells Explaining, question and answer teaching method
14 Organism cloning, transgenic creatures Explaining, question and answer teaching method
15 The impacts and future of recombinant DNA technology and genetic engineering and student presentations Explaining, question and answer teaching method
16 final exam
Recommend Course Book / Supplementary Book/Reading
1 An Introduction to Genetic Engineering, DST. Nicholl, 3. Edition, Cambridge University Press, 2008.
Required Course instruments and materials
Text Book

Assessment Methods
Type of Assessment Week Hours Weight(%)
mid-term exam 8 2 40
Other assessment methods
1.Oral Examination
2.Quiz
3.Laboratory exam
4.Presentation
5.Report
6.Workshop
7.Performance Project
8.Term Paper
9.Project
final exam 16 2 60

Student Work Load
Type of Work Weekly Hours Number of Weeks Work Load
Weekly Course Hours (Theoretical+Practice) 2 14 28
Outside Class
       a) Reading 2 6 12
       b) Search in internet/Library 3 6 18
       c) Performance Project 0
       d) Prepare a workshop/Presentation/Report 2 5 10
       e) Term paper/Project 0
Oral Examination 0
Quiz 0
Laboratory exam 0
Own study for mid-term exam 2 5 10
mid-term exam 1 1 1
Own study for final exam 2 5 10
final exam 1 1 1
0
0
Total work load; 90