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| Year/Semester of Study | 3 / Fall Semester | ||||
| Level of Course | 1st Cycle Degree Programme | ||||
| Type of Course | Optional | ||||
| Department | MOLECULAR BIOLOGY AND GENETICS | ||||
| Pre-requisities and Co-requisites | None | ||||
| Mode of Delivery | Face to Face | ||||
| Teaching Period | 14 Weeks | ||||
| Name of Lecturer | ÜMİT KUMBIÇAK (umitkumbicak@nevsehir.edu.tr) | ||||
| Name of Lecturer(s) | |||||
| Language of Instruction | Turkish | ||||
| Work Placement(s) | None | ||||
| Objectives of the Course | |||||
| To teach the areas of genetic engineering techniques, the products produced by genetic engineering, and the products obtained from genetically modified organisms. | |||||
| Learning Outcomes | PO | MME | |
| The students who succeeded in this course: | |||
| LO-1 | Can explain the applications and techniques of genetic engineering |
PO-1 Obtain basic knowledge of Biology PO-5 Explain transmission of genetic background in organisms and populations PO-8 Develop an awareness of continuous learning in relation with modern technology |
Examination Presentation |
| LO-2 | Can explain the gene cloning steps and strategies |
PO-9 Find out new methods to improve his/her knowledge PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics PO-14 Have knowledge about the main research interests of the molecular biology and genetics |
Examination Presentation |
| LO-3 | Can explain the methods of obtaining transgenic creatures |
PO-11 Apply biological concepts to personal, social, economical, technological and ethical issues PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics PO-14 Have knowledge about the main research interests of the molecular biology and genetics |
Examination Presentation |
| PO: Programme Outcomes MME:Method of measurement & Evaluation |
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| Course Contents | ||
| Genetic engineering areas, and strategies of cloning steps , the construction of genomic DNA and cDNA libraries , siRNA and miRNA technology, cloning organisms and transgenic organisms, cloning and purification of protein. | ||
| Weekly Course Content | ||
| Week | Subject | Learning Activities and Teaching Methods |
| 1 | Introduction to genetic engineering, definition of concepts and application areas of genetic engineering | Explaining, question and answer teaching method |
| 2 | Structure of the genome, replication, transcription and translation | Explaining, question and answer teaching method |
| 3 | Enzymes and molecular biological methods used in genetic engineering | Explaining, question and answer teaching method |
| 4 | Vectors used in genetic engineering and their properties | Explaining, question and answer teaching method |
| 5 | Host organism types and gene transfer methods | Explaining, question and answer teaching method |
| 6 | Gene cloning techniques, clone detection methods | Explaining, question and answer teaching method |
| 7 | Basic principles and usage areas of nucleic acid hybridization methods | Explaining, question and answer teaching method |
| 8 | mid-term exam | |
| 9 | DNA isolation, Genomic DNA and cDNA libraries | Explaining, question and answer teaching method |
| 10 | Genome analysis and human genome project | Explaining, question and answer teaching method |
| 11 | Recombinant DNA recovery and products obtained by Recombinant DNA technology (protein, vaccine and hormone production) | Explaining, question and answer teaching method |
| 12 | siRNA and miRNA technology and gene therapy applications and student presentations | Explaining, question and answer teaching method |
| 13 | Genetic applications in somatic cells. Protoplast isolation, fusion and somatic hybrids, reconstructed cells | Explaining, question and answer teaching method |
| 14 | Organism cloning, transgenic creatures | Explaining, question and answer teaching method |
| 15 | The impacts and future of recombinant DNA technology and genetic engineering and student presentations | Explaining, question and answer teaching method |
| 16 | final exam | |
| Recommend Course Book / Supplementary Book/Reading | ||
| 1 | An Introduction to Genetic Engineering, DST. Nicholl, 3. Edition, Cambridge University Press, 2008. | |
| Required Course instruments and materials | ||
| Text Book | ||
| Assessment Methods | |||
| Type of Assessment | Week | Hours | Weight(%) |
| mid-term exam | 8 | 2 | 40 |
| Other assessment methods | |||
| 1.Oral Examination | |||
| 2.Quiz | |||
| 3.Laboratory exam | |||
| 4.Presentation | |||
| 5.Report | |||
| 6.Workshop | |||
| 7.Performance Project | |||
| 8.Term Paper | |||
| 9.Project | |||
| final exam | 16 | 2 | 60 |
| Student Work Load | |||
| Type of Work | Weekly Hours | Number of Weeks | Work Load |
| Weekly Course Hours (Theoretical+Practice) | 2 | 14 | 28 |
| Outside Class | |||
| a) Reading | 2 | 6 | 12 |
| b) Search in internet/Library | 3 | 6 | 18 |
| c) Performance Project | 0 | ||
| d) Prepare a workshop/Presentation/Report | 2 | 5 | 10 |
| e) Term paper/Project | 0 | ||
| Oral Examination | 0 | ||
| Quiz | 0 | ||
| Laboratory exam | 0 | ||
| Own study for mid-term exam | 2 | 5 | 10 |
| mid-term exam | 1 | 1 | 1 |
| Own study for final exam | 2 | 5 | 10 |
| final exam | 1 | 1 | 1 |
| 0 | |||
| 0 | |||
| Total work load; | 90 | ||