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Year/Semester of Study | 1 / Spring Semester | ||||
Level of Course | 1st Cycle Degree Programme | ||||
Type of Course | Compulsory | ||||
Department | MOLECULAR BIOLOGY AND GENETICS | ||||
Pre-requisities and Co-requisites | None | ||||
Mode of Delivery | Face to Face | ||||
Teaching Period | 14 Weeks | ||||
Name of Lecturer | ÜMİT KUMBIÇAK (umitkumbicak@nevsehir.edu.tr) | ||||
Name of Lecturer(s) | |||||
Language of Instruction | Turkish | ||||
Work Placement(s) | None | ||||
Objectives of the Course | |||||
The aim of this course is to gain knowledge and skills about separation, purification and analysis methods used in laboratory. |
Learning Outcomes | PO | MME | |
The students who succeeded in this course: | |||
LO-1 | can explain the basic concepts of separation and purification processes. |
PO-1 Obtain basic knowledge of Biology PO-6 Develop an understanding of nature and history of scientific thought PO-7 Work effectively in multi-disciplinary research teams PO-8 Develop an awareness of continuous learning in relation with modern technology PO-9 Find out new methods to improve his/her knowledge PO-10 Demonstrate sufficiency in English to follow literature, present technical projects and write articles PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics PO-14 Have knowledge about the main research interests of the molecular biology and genetics |
Examination |
LO-2 | can explain basic concepts related to spectrophotometric, turbidimetric and fluorometric methods. |
PO-1 Obtain basic knowledge of Biology PO-7 Work effectively in multi-disciplinary research teams PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics PO-14 Have knowledge about the main research interests of the molecular biology and genetics PO-17 Survey, analyze and interpret the experimental results and to reach a scientific result using the available scientific data. |
Examination |
LO-3 | can explain basic concepts of gravimetric analysis and volumetric analysis. |
PO-14 Have knowledge about the main research interests of the molecular biology and genetics PO-17 Survey, analyze and interpret the experimental results and to reach a scientific result using the available scientific data. |
Examination |
LO-4 | explain the basic concepts of electrophoretic and chromatographic methods. |
PO-1 Obtain basic knowledge of Biology PO-14 Have knowledge about the main research interests of the molecular biology and genetics |
Examination |
LO-5 | can explain the basic concepts related to nucleic acid based methods and protein determination methods. |
PO-5 Explain transmission of genetic background in organisms and populations PO-6 Develop an understanding of nature and history of scientific thought PO-8 Develop an awareness of continuous learning in relation with modern technology PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics PO-14 Have knowledge about the main research interests of the molecular biology and genetics PO-17 Survey, analyze and interpret the experimental results and to reach a scientific result using the available scientific data. |
Examination |
PO: Programme Outcomes MME:Method of measurement & Evaluation |
Course Contents | ||
Separation purification and analysis processes, evaporation, extraction, distillation, sublimation, crystallization, precipitation, filtration, gravimetric analysis and volumetric analysis, chromatographic methods and types, application areas of chromatographic methods, spectrophotometer, spectrophotometer types and usage areas, turbidimetric and fluorometric methods, Basic principles of electrophoretic methods, application areas of electrophoretic methods, nucleic acid based methods, protein determination methods | ||
Weekly Course Content | ||
Week | Subject | Learning Activities and Teaching Methods |
1 | Basic applications applied in the laboratory | Explanation Method |
2 | Separation and purification processes | Explanation Method |
3 | Separation and purification processes | Explanation Method |
4 | Gravimetric analysis and volumetric analysis | Explanation Method |
5 | Instrumental analysis methods | Explanation Method |
6 | Optical and spectrophotometric methods | Explanation Method |
7 | Usage areas of spectroscopic methods | Explanation Method |
8 | mid-term exam | |
9 | Chromatographic analysis methods | Explanation Method |
10 | Current applications of chromatographic methods | Explanation Method |
11 | Electrophoretic methods and basic principles | Explanation Method |
12 | Application areas of electrophoretic methods | Explanation Method |
13 | Nucleic acid–based methods of analysis | Explanation Method |
14 | biochemical and immunochemical test methods | Explanation Method |
15 | Protein determination methods | Explanation Method |
16 | final exam | |
Recommend Course Book / Supplementary Book/Reading | ||
1 | Gündüz, T. 2015 "Kromatografi ve Elektroforez" | |
2 | Bağ, H., Kendüzler, E. "Genel Kimya III (Analitik Kimya) " | |
3 | Felicia Y.H. Wu, Cheng-Wen Wu. Structure and Function of Nucleic Acids and Proteins | |
Required Course instruments and materials | ||
Textbooks and course presentations |
Assessment Methods | |||
Type of Assessment | Week | Hours | Weight(%) |
mid-term exam | 8 | 2 | 40 |
Other assessment methods | |||
1.Oral Examination | |||
2.Quiz | |||
3.Laboratory exam | |||
4.Presentation | |||
5.Report | |||
6.Workshop | |||
7.Performance Project | |||
8.Term Paper | |||
9.Project | |||
final exam | 16 | 2 | 40 |
Student Work Load | |||
Type of Work | Weekly Hours | Number of Weeks | Work Load |
Weekly Course Hours (Theoretical+Practice) | 3 | 14 | 42 |
Outside Class | |||
a) Reading | 2 | 14 | 28 |
b) Search in internet/Library | 1 | 14 | 14 |
c) Performance Project | 0 | ||
d) Prepare a workshop/Presentation/Report | 0 | ||
e) Term paper/Project | 0 | ||
Oral Examination | 0 | ||
Quiz | 0 | ||
Laboratory exam | 0 | ||
Own study for mid-term exam | 2 | 8 | 16 |
mid-term exam | 2 | 1 | 2 |
Own study for final exam | 2 | 8 | 16 |
final exam | 2 | 1 | 2 |
0 | |||
0 | |||
Total work load; | 120 |