Nevşehir Hacı Bektaş Veli University Course Catalogue

Information Of Programmes

FACULTY OF LETTERS & SCIENCE / MBG 108 - MOLECULAR BIOLOGY AND GENETICS

Code: MBG 108 Course Title: MODERN LABORATORY TECHNIQUES II Theoretical+Practice: 3+0 ECTS: 4
Year/Semester of Study 1 / Spring Semester
Level of Course 1st Cycle Degree Programme
Type of Course Compulsory
Department MOLECULAR BIOLOGY AND GENETICS
Pre-requisities and Co-requisites None
Mode of Delivery Face to Face
Teaching Period 14 Weeks
Name of Lecturer ÜMİT KUMBIÇAK (umitkumbicak@nevsehir.edu.tr)
Name of Lecturer(s)
Language of Instruction Turkish
Work Placement(s) None
Objectives of the Course
The aim of this course is to gain knowledge and skills about separation, purification and analysis methods used in laboratory.

Learning Outcomes PO MME
The students who succeeded in this course:
LO-1 can explain the basic concepts of separation and purification processes. PO-1 Obtain basic knowledge of Biology
PO-6 Develop an understanding of nature and history of scientific thought
PO-7 Work effectively in multi-disciplinary research teams
PO-8 Develop an awareness of continuous learning in relation with modern technology
PO-9 Find out new methods to improve his/her knowledge
PO-10 Demonstrate sufficiency in English to follow literature, present technical projects and write articles
PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics
PO-14 Have knowledge about the main research interests of the molecular biology and genetics
Examination
LO-2 can explain basic concepts related to spectrophotometric, turbidimetric and fluorometric methods. PO-1 Obtain basic knowledge of Biology
PO-7 Work effectively in multi-disciplinary research teams
PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics
PO-14 Have knowledge about the main research interests of the molecular biology and genetics
PO-17 Survey, analyze and interpret the experimental results and to reach a scientific result using the available scientific data.
Examination
LO-3 can explain basic concepts of gravimetric analysis and volumetric analysis. PO-14 Have knowledge about the main research interests of the molecular biology and genetics
PO-17 Survey, analyze and interpret the experimental results and to reach a scientific result using the available scientific data.
Examination
LO-4 explain the basic concepts of electrophoretic and chromatographic methods. PO-1 Obtain basic knowledge of Biology
PO-14 Have knowledge about the main research interests of the molecular biology and genetics
Examination
LO-5 can explain the basic concepts related to nucleic acid based methods and protein determination methods. PO-5 Explain transmission of genetic background in organisms and populations
PO-6 Develop an understanding of nature and history of scientific thought
PO-8 Develop an awareness of continuous learning in relation with modern technology
PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics
PO-14 Have knowledge about the main research interests of the molecular biology and genetics
PO-17 Survey, analyze and interpret the experimental results and to reach a scientific result using the available scientific data.
Examination
PO: Programme Outcomes
MME:Method of measurement & Evaluation

Course Contents
Separation purification and analysis processes, evaporation, extraction, distillation, sublimation, crystallization, precipitation, filtration, gravimetric analysis and volumetric analysis, chromatographic methods and types, application areas of chromatographic methods, spectrophotometer, spectrophotometer types and usage areas, turbidimetric and fluorometric methods, Basic principles of electrophoretic methods, application areas of electrophoretic methods, nucleic acid based methods, protein determination methods
Weekly Course Content
Week Subject Learning Activities and Teaching Methods
1 Basic applications applied in the laboratory Explanation Method
2 Separation and purification processes Explanation Method
3 Separation and purification processes Explanation Method
4 Gravimetric analysis and volumetric analysis Explanation Method
5 Instrumental analysis methods Explanation Method
6 Optical and spectrophotometric methods Explanation Method
7 Usage areas of spectroscopic methods Explanation Method
8 mid-term exam
9 Chromatographic analysis methods Explanation Method
10 Current applications of chromatographic methods Explanation Method
11 Electrophoretic methods and basic principles Explanation Method
12 Application areas of electrophoretic methods Explanation Method
13 Nucleic acid–based methods of analysis Explanation Method
14 biochemical and immunochemical test methods Explanation Method
15 Protein determination methods Explanation Method
16 final exam
Recommend Course Book / Supplementary Book/Reading
1 Gündüz, T. 2015 "Kromatografi ve Elektroforez"
2 Bağ, H., Kendüzler, E. "Genel Kimya III (Analitik Kimya) "
3 Felicia Y.H. Wu, Cheng-Wen Wu. Structure and Function of Nucleic Acids and Proteins
Required Course instruments and materials
Textbooks and course presentations

Assessment Methods
Type of Assessment Week Hours Weight(%)
mid-term exam 8 2 40
Other assessment methods
1.Oral Examination
2.Quiz
3.Laboratory exam
4.Presentation
5.Report
6.Workshop
7.Performance Project
8.Term Paper
9.Project
final exam 16 2 40

Student Work Load
Type of Work Weekly Hours Number of Weeks Work Load
Weekly Course Hours (Theoretical+Practice) 3 14 42
Outside Class
       a) Reading 2 14 28
       b) Search in internet/Library 1 14 14
       c) Performance Project 0
       d) Prepare a workshop/Presentation/Report 0
       e) Term paper/Project 0
Oral Examination 0
Quiz 0
Laboratory exam 0
Own study for mid-term exam 2 8 16
mid-term exam 2 1 2
Own study for final exam 2 8 16
final exam 2 1 2
0
0
Total work load; 120