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Year/Semester of Study | 3 / Fall Semester | ||||
Level of Course | 1st Cycle Degree Programme | ||||
Type of Course | Optional | ||||
Department | MOLECULAR BIOLOGY AND GENETICS | ||||
Pre-requisities and Co-requisites | None | ||||
Mode of Delivery | Face to Face | ||||
Teaching Period | 14 Weeks | ||||
Name of Lecturer | ZÜBEYDE KUMBIÇAK (zkumbicak@nevsehir.edu.tr) | ||||
Name of Lecturer(s) | |||||
Language of Instruction | Turkish | ||||
Work Placement(s) | None | ||||
Objectives of the Course | |||||
The aim of this course is to gain basic knowledge and skills about genetic methods. |
Learning Outcomes | PO | MME | |
The students who succeeded in this course: | |||
LO-1 | can explain the basic concepts related to genetic methods. |
PO-1 Obtain basic knowledge of Biology PO-5 Explain transmission of genetic background in organisms and populations PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics PO-14 Have knowledge about the main research interests of the molecular biology and genetics |
Examination |
PO: Programme Outcomes MME:Method of measurement & Evaluation |
Course Contents | ||
DNA isolation methods and calculations, RNA isolation methods and their calculations, restriction enzymes, polymerase chain reaction, hybridization of nucleic acids, Southern and Northern Blot analyzes, DNA sequencing, Allel-specific oligonucleotide analysis, microarray analysis, short tandem repeats (STR), STR analysis, evaluation of STR profiles, classical cytogenetic methods, molecular cytogenetic methods | ||
Weekly Course Content | ||
Week | Subject | Learning Activities and Teaching Methods |
1 | DNA isolation methods and calculations | Explanation Method |
2 | RNA isolation methods and calculations | Explanation Method |
3 | Restriction enzymes | Explanation Method |
4 | Polymerase chain reaction | Explanation Method |
5 | Hybridization of nucleic acids, Southern and Northern Blot analyzes | Explanation Method |
6 | DNA sequence analysis | Explanation Method |
7 | Allele-specific oligonucleotide analysis | Explanation Method |
8 | mid-term exam | |
9 | Microarray Analysis | Explanation Method |
10 | Short tandem repeats (STR) analysis | Explanation Method |
11 | Evaluation of STR profiles | Explanation Method |
12 | Classical cytogenetic methods | Explanation Method |
13 | Molecular cytogenetic methods | Explanation Method |
14 | Flow karyotyping Fluorescence in situ hybridization (FISH) | Explanation Method |
15 | Fiber FISH Multicolor-FISH | Explanation Method |
16 | final exam | |
Recommend Course Book / Supplementary Book/Reading | ||
1 | Topaktaş, M. "Moleküler Genetik" | |
2 | Temizkan, G., Arda, N. "Temel ve İleri Moleküler Biyoloji Yöntemleri Genomik ve Proteomik Analizler" | |
Required Course instruments and materials | ||
textbook and course presentations |
Assessment Methods | |||
Type of Assessment | Week | Hours | Weight(%) |
mid-term exam | 8 | 1 | 40 |
Other assessment methods | |||
1.Oral Examination | |||
2.Quiz | |||
3.Laboratory exam | |||
4.Presentation | |||
5.Report | |||
6.Workshop | |||
7.Performance Project | |||
8.Term Paper | |||
9.Project | |||
final exam | 16 | 1 | 60 |
Student Work Load | |||
Type of Work | Weekly Hours | Number of Weeks | Work Load |
Weekly Course Hours (Theoretical+Practice) | 2 | 14 | 28 |
Outside Class | |||
a) Reading | 2 | 6 | 12 |
b) Search in internet/Library | 3 | 6 | 18 |
c) Performance Project | 2 | 5 | 10 |
d) Prepare a workshop/Presentation/Report | 0 | ||
e) Term paper/Project | 0 | ||
Oral Examination | 0 | ||
Quiz | 0 | ||
Laboratory exam | 0 | ||
Own study for mid-term exam | 2 | 5 | 10 |
mid-term exam | 1 | 1 | 1 |
Own study for final exam | 2 | 5 | 10 |
final exam | 1 | 1 | 1 |
0 | |||
0 | |||
Total work load; | 90 |