Learning Outcomes |
PO |
MME |
The students who succeeded in this course: |
|
|
LO-1 |
Can have information about academic studies or industrial / production applications, which are two areas in which biological separation methods can be taught in future professions by teaching on laboratory scale and industrial scale. |
PO-11 Explains the basic functions of scientific processes, critically discusses and solves problems using appropriate statistical and computational methods, chooses contemporary experimental and theoretical approaches that will ultimately lead to the solution of molecular biology and genetic research problems
|
Examination Presentation |
LO-2 |
Can learn the biological applications of separation processes, one of the important work areas of chemical engineering, and to be knowledgeable about the most important application fields of health and environment |
PO-4 Being ethical and responsible individuals who are sensitive to social and universal values in the field of Molecular Biology and genetics, who look after the priority areas of the country, research and produce and contribute to these issues
|
Examination Presentation |
LO-3 |
Can comprehend the modern structure of chemical engineering open to interdisciplinary interactions by recognizing biological processes |
PO-9 Recognizes and explains the molecular biology and genetics of the problems of other disciplines
|
Examination Presentation |
PO: Programme Outcomes MME:Method of measurement & Evaluation |
Course Contents |
Characteristics of biological systems. Separation methods in biological production processes. Extraction / enrichment of products in biological production processes by cell / protein / enzyme isolation, precipitation, centrifugation, extraction, adsorption, filtration, chromatography, crystallization, electrophoresis, reverse osmosis methods and membrane based methods. Comparison of laboratory scale biodegradation systems with industrial biodegradation systems. Examination and interpretation of the articles belonging to the original scientific studies related to the topic from the literature. |
Weekly Course Content |
Week |
Subject |
Learning Activities and Teaching Methods |
1 |
General definitions of basic, purposeful and used methods of biosperation techniques |
Lecture, discussion, question and answer |
2 |
Characteristics of biological production systems, basic microbiology concepts |
Lecture, discussion, question and answer |
3 |
Usage of biological systems in industrial production processes: current use areas (dairy products, wine, agriculture, textile, medicine, waste water cleaning etc.) and environmentally friendly potential use areas (bioethanol, biodiesel, biopolymers etc.) |
Lecture, discussion, question and answer |
4 |
Cell / protein / enzyme isolation techniques: mechanical, chemical and biological methods |
Lecture, discussion, question and answer |
5 |
Separation by sedimentation: organic solvents, salts and precipitation mechanism Separation by centrifugation: Laboratory scale, preparative scale and ultrafiltration |
Lecture, discussion, question and answer |
6 |
Separation by extraction: solvent systems, aqueous two phase separation technique, continuous and continuous systems, supercritical extraction method |
Lecture, discussion, question and answer |
7 |
Separation by adsorption: Adsorbents, separation mechanisms, intermittent and packed bed separation systems |
Lecture, discussion, question and answer |
8 |
mid-term exam |
|
9 |
Filtrasyon ile ayırma: Kullanılan ekipmanlar, ayırma mekanizması |
Lecture, discussion, question and answer |
10 |
Chromatographic separation: Liquid and gas chromatography methods, column systems, TLC, HPLC, FPLC, gel filtration, ion exchange, hydrophobic chromatography techniques |
Lecture, discussion, question and answer |
11 |
Separation by crystallization |
Lecture, discussion, question and answer |
12 |
Separation by electrophoresis: Gel and free flow techniques |
Lecture, discussion, question and answer |
13 |
Membrane separation techniques: Equipment used, dialysis, membrane chromatography, reverse osmosis |
Lecture, discussion, question and answer |
14 |
Oral presentations, discussions |
Lecture, discussion, question and answer |
15 |
Oral presentations, discussions |
Lecture, discussion, question and answer |
16 |
final exam |
|
Recommend Course Book / Supplementary Book/Reading |
1 |
1. Ghosh, R., Principles of Bioseparations Engineering, World Scientific Publishing, Singapore¬, 2006. YARDIMCI KİTAPLAR: 1. Desai, M.A., Downstream Processing of Proteins, Humana Press, Totowa, New Jersey, 2000. |
2 |
2. Sham, H., Biotechnology, 2. Basım, Sayı 1: Biological Fundamentals, VCH, Almanya, 1993. |
Required Course instruments and materials |
projection |