Nevşehir Hacı Bektaş Veli University Course Catalogue
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| Year/Semester of Study | 1 / Fall Semester | ||||
| Level of Course | 2nd Cycle Degree Programme | ||||
| Type of Course | Optional | ||||
| Department | MONECULER BIOLOGY AND GENETICS | ||||
| Pre-requisities and Co-requisites | None | ||||
| Mode of Delivery | Face to Face | ||||
| Teaching Period | 14 Weeks | ||||
| Name of Lecturer | NAŞİT İĞCİ (nasitigci@nevsehir.edu.tr) | ||||
| Name of Lecturer(s) | |||||
| Language of Instruction | Turkish | ||||
| Work Placement(s) | None | ||||
| Objectives of the Course | |||||
| The aim of this course is to give information about basic laboratory methods used in molecular biology. | |||||
| Learning Outcomes | PO | MME | |
| The students who succeeded in this course: | |||
| LO-1 | Can explain the basic methods that are used in protein and enzyme engineering. |
PO-1 Obtain basic knowledge of Molecular Biology PO-2 Have information about current topics in molecular biology and genetics PO-3 Ability to identify problems in Molecular Biology and genetics, to establish hypotheses for solving problems and to be able to solve hypothesis using various observational and experimental methods PO-6 Ability to independently carry out an activity that requires expertise in the field PO-7 Investigating the phenotypic effects of genes and acquiring the ability to use this effect in animal and plant breeding areas PO-8 Ability to participate in interdisciplinary studies in the fields of education, industry, agriculture, health, environment, energy and biotechnology PO-9 Recognizes and explains the molecular biology and genetics of the problems of other disciplines PO-13 Scientific developments contribute to research and development |
Examination Presentation |
| LO-2 | Can give information about genomics and proteomics and give new ideas about those. |
PO-1 Obtain basic knowledge of Molecular Biology PO-2 Have information about current topics in molecular biology and genetics PO-3 Ability to identify problems in Molecular Biology and genetics, to establish hypotheses for solving problems and to be able to solve hypothesis using various observational and experimental methods PO-6 Ability to independently carry out an activity that requires expertise in the field PO-7 Investigating the phenotypic effects of genes and acquiring the ability to use this effect in animal and plant breeding areas PO-8 Ability to participate in interdisciplinary studies in the fields of education, industry, agriculture, health, environment, energy and biotechnology PO-9 Recognizes and explains the molecular biology and genetics of the problems of other disciplines PO-13 Scientific developments contribute to research and development |
Examination Presentation |
| LO-3 | can hold a view about genetically modified organisms and explain their intended use on agriculture, medicine and biotechnology. |
PO-1 Obtain basic knowledge of Molecular Biology PO-2 Have information about current topics in molecular biology and genetics PO-3 Ability to identify problems in Molecular Biology and genetics, to establish hypotheses for solving problems and to be able to solve hypothesis using various observational and experimental methods PO-6 Ability to independently carry out an activity that requires expertise in the field PO-7 Investigating the phenotypic effects of genes and acquiring the ability to use this effect in animal and plant breeding areas PO-8 Ability to participate in interdisciplinary studies in the fields of education, industry, agriculture, health, environment, energy and biotechnology PO-9 Recognizes and explains the molecular biology and genetics of the problems of other disciplines PO-13 Scientific developments contribute to research and development |
Examination Presentation |
| LO-4 | Can apply basic DNA and protein methods. |
PO-1 Obtain basic knowledge of Molecular Biology PO-2 Have information about current topics in molecular biology and genetics PO-3 Ability to identify problems in Molecular Biology and genetics, to establish hypotheses for solving problems and to be able to solve hypothesis using various observational and experimental methods PO-6 Ability to independently carry out an activity that requires expertise in the field PO-7 Investigating the phenotypic effects of genes and acquiring the ability to use this effect in animal and plant breeding areas PO-8 Ability to participate in interdisciplinary studies in the fields of education, industry, agriculture, health, environment, energy and biotechnology PO-9 Recognizes and explains the molecular biology and genetics of the problems of other disciplines PO-13 Scientific developments contribute to research and development |
Examination Presentation |
| PO: Programme Outcomes MME:Method of measurement & Evaluation |
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| Course Contents | ||
| DNA and protein isolation and purification, determination of DNA and protein concentration, DNA and protein electrophoresis, gel staining methods, hybridization techniques, antibody based methods, enzymatic activity determination methods, genome and proteome analysis, protein and enzyme engineering, genetically modified organisms, gene therapy | ||
| Weekly Course Content | ||
| Week | Subject | Learning Activities and Teaching Methods |
| 1 | DNA and protein isolation. | Lecture, Discussion, Question and Answer, Literature Search |
| 2 | Concentration of solutions containing biological macromolecules. | Lecture, Discussion, Question and Answer, Literature Search |
| 3 | Determination of DNA and protein concentration. | Lecture, Discussion, Question and Answer, Literature Search |
| 4 | Nucleic acid electrophoresis and gel staining. | Lecture, Discussion, Question and Answer, Literature Search |
| 5 | Protein electrophoresis and gel staining. | Lecture, Discussion, Question and Answer, Literature Search |
| 6 | Membrane transfer and detection (blotting) methods. | Lecture, Discussion, Question and Answer, Literature Search |
| 7 | Polymerase Chain Reaction (PCR). | Lecture, Discussion, Question and Answer, Literature Search |
| 8 | mid-term exam | |
| 9 | DNA sequencing methods. | Lecture, Discussion, Question and Answer, Literature Search |
| 10 | Protein purification methods. | Lecture, Discussion, Question and Answer, Literature Search |
| 11 | Applications of mass spectrometry in molecular biology. | Lecture, Discussion, Question and Answer, Literature Search |
| 12 | Protein sequencing methods. | Lecture, Discussion, Question and Answer, Literature Search |
| 13 | Enzyme activity determination methods. | Lecture, Discussion, Question and Answer, Literature Search |
| 14 | Determination of DNA-protein, protein-protein interactions. | Lecture, Discussion, Question and Answer, Literature Search |
| 15 | Gene and protein engineering. | Lecture, Discussion, Question and Answer, Literature Search |
| 16 | final exam | |
| Recommend Course Book / Supplementary Book/Reading | ||
| 1 | Frederick M. Ausubel, Current Protocols in Molecular Biology, John Wiley & Sons Inc, 2003. | |
| 2 | Mary C. Phelan Current Protocols in Cell Biology, John Wiley & Sons, Inc, 1998. | |
| 3 | Temizkan G, Arda N. (Ed.). Temel ve İleri Moleküler Biyoloji Yöntemleri Genomik ve Protemik Analizler. Nobel Tıp Kitabevi, 2018. | |
| Required Course instruments and materials | ||
| Lecture notes, books, related articles, computer | ||
| Assessment Methods | |||
| Type of Assessment | Week | Hours | Weight(%) |
| mid-term exam | 8 | 1 | 20 |
| Other assessment methods | |||
| 1.Oral Examination | |||
| 2.Quiz | |||
| 3.Laboratory exam | |||
| 4.Presentation | 15 | 1 | 30 |
| 5.Report | |||
| 6.Workshop | |||
| 7.Performance Project | |||
| 8.Term Paper | |||
| 9.Project | |||
| final exam | 16 | 1 | 50 |
| Student Work Load | |||
| Type of Work | Weekly Hours | Number of Weeks | Work Load |
| Weekly Course Hours (Theoretical+Practice) | 3 | 14 | 42 |
| Outside Class | |||
| a) Reading | 3 | 14 | 42 |
| b) Search in internet/Library | 3 | 14 | 42 |
| c) Performance Project | 0 | ||
| d) Prepare a workshop/Presentation/Report | 2 | 15 | 30 |
| e) Term paper/Project | 0 | ||
| Oral Examination | 0 | ||
| Quiz | 0 | ||
| Laboratory exam | 0 | ||
| Own study for mid-term exam | 1 | 7 | 7 |
| mid-term exam | 1 | 1 | 1 |
| Own study for final exam | 2 | 7 | 14 |
| final exam | 2 | 1 | 2 |
| 0 | |||
| 0 | |||
| Total work load; | 180 | ||