Nevşehir Hacı Bektaş Veli University Course Catalogue

Information Of Programmes

FACULTY OF LETTERS & SCIENCE / MBG 433 - MOLECULAR BIOLOGY AND GENETICS

Code: MBG 433 Course Title: THERAPEUTIC PROTEINS Theoretical+Practice: 2+0 ECTS: 3
Year/Semester of Study 4 / Fall Semester
Level of Course 1st Cycle Degree Programme
Type of Course Optional
Department MOLECULAR BIOLOGY AND GENETICS
Pre-requisities and Co-requisites None
Mode of Delivery Face to Face
Teaching Period 14 Weeks
Name of Lecturer NAŞİT İĞCİ (nasitigci@nevsehir.edu.tr)
Name of Lecturer(s) CEVAHİR ALTINKAYNAK,
Language of Instruction Turkish
Work Placement(s) None
Objectives of the Course
The aim of this course is to provide information on the identification, production, purification, characterization and mechanism of action of molecules used as therapeutic agents using protein engineering approaches.

Learning Outcomes PO MME
The students who succeeded in this course:
LO-1 can explain about the structure, classification, function and aggregation of proteins. PO-3 Identify structure-function relationships in cells and organisms
PO-6 Develop an understanding of nature and history of scientific thought
PO-9 Find out new methods to improve his/her knowledge
PO-16 Explain the scientific findings clearly and efficiently both in written and verbal communications.
 		Examination
LO-2 can explain the separation, purification, characterization and chemical modifications of proteins. PO-6 Develop an understanding of nature and history of scientific thought
PO-9 Find out new methods to improve his/her knowledge
PO-16 Explain the scientific findings clearly and efficiently both in written and verbal communications.
 		Examination
LO-3 can explain the methods of protein engineering. PO-6 Develop an understanding of nature and history of scientific thought
PO-8 Develop an awareness of continuous learning in relation with modern technology
PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics
 		Examination
LO-4 can explain therapeutic proteins and the strategies of production. PO-11 Apply biological concepts to personal, social, economical, technological and ethical issues
PO-16 Explain the scientific findings clearly and efficiently both in written and verbal communications.
 		Examination
LO-5 can explain about the definition of antibody, monoclonal antibody and antibody fragments and mechanism of action PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics
PO-16 Explain the scientific findings clearly and efficiently both in written and verbal communications.
 		Examination
LO-6 can explain biosimilars and its uses in clinical practice. PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics
PO-16 Explain the scientific findings clearly and efficiently both in written and verbal communications.
 		Examination
LO-7 can explain about venom peptides. PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics
PO-16 Explain the scientific findings clearly and efficiently both in written and verbal communications.
 		Examination
PO: Programme Outcomes
MME:Method of measurement & Evaluation

Course Contents
Protein structure, classification, function, protein purification and characterization, chemical modification, protein engineering methods, classification of therapeutic proteins (EPO, insulin, interferon alpha and beta, blood factors, colony stimulating factors, interleukins, growth factors, monoclonal antibodies), recombinant production strategies, antibody structure and functions, monoclonal antibody production technology, biosimilars and clinical uses, venom peptides.
Weekly Course Content
Week Subject Learning Activities and Teaching Methods
1 Classification of Proteins and its Functions and Structures Expression Question-Answer
2 Function and aggregation of proteins Expression Question-Answer
3 Separation, purification and characterization of proteins Expression Question-Answer
4 Chemical Modification of Proteins Expression Question-Answer
5 The aims of protein engineering Expression Question-Answer
6 Protein engineering methods Expression Question-Answer
7 Classification of therapeutic proteins Expression Question-Answer
8 mid-term exam
9 Therapeutic proteins and the strategies of recombinant production Expression Question-Answer
10 Antibody structure and function Expression Question-Answer
11 Monoclonal antibody production methods and mechanism of action Expression Question-Answer
12 Engineering antibody therapeutics Expression Question-Answer
13 Biosimilars Expression Question-Answer
14 Problems to be encountered in clinical practice in biosimilars Expression Question-Answer
15 Venom Peptides Expression Question-Answer
16 final exam
Recommend Course Book / Supplementary Book/Reading
1 Therapeutic Proteins-Vladimir Voynov-Humana Press
2 Biyoteknolojiye Giriş- William J. Thieman/Michael A. Palladino
3 Güncel Biyoteknoloji ve Uygulamaları-Münis Dündar/Haydar Bağış
4 Protein Mühendisliği- Azmi telefoncu/Ö.İrfan Küfrevioğlu/Figen Zihnioğlu/Ali Kılınç
Required Course instruments and materials

Assessment Methods
Type of Assessment Week Hours Weight(%)
mid-term exam 8 1 40
Other assessment methods
1.Oral Examination
2.Quiz
3.Laboratory exam
4.Presentation
5.Report
6.Workshop
7.Performance Project
8.Term Paper
9.Project
final exam 16 1 60

Student Work Load
Type of Work Weekly Hours Number of Weeks Work Load
Weekly Course Hours (Theoretical+Practice) 2 14 28
Outside Class
       a) Reading 2 14 28
       b) Search in internet/Library 2 8 16
       c) Performance Project 0
       d) Prepare a workshop/Presentation/Report 0
       e) Term paper/Project 0
Oral Examination 0
Quiz 0
Laboratory exam 0
Own study for mid-term exam 4 2 8
mid-term exam 1 1 1
Own study for final exam 4 2 8
final exam 1 1 1
0
0
Total work load; 90