Nevşehir Hacı Bektaş Veli University Course Catalogue
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| Year/Semester of Study | 1 / Spring Semester | ||||
| Level of Course | 2nd Cycle Degree Programme | ||||
| Type of Course | Optional | ||||
| Department | CHEMISTRY (MASTER'S DEGREE) | ||||
| Pre-requisities and Co-requisites | None | ||||
| Mode of Delivery | Face to Face | ||||
| Teaching Period | 14 Weeks | ||||
| Name of Lecturer | BAHAR TUBA FINDIK (btfindik@nevsehir.edu.tr) | ||||
| Name of Lecturer(s) | BAHAR TUBA FINDIK, | ||||
| Language of Instruction | Turkish | ||||
| Work Placement(s) | None | ||||
| Objectives of the Course | |||||
| Research Techniques in Biochemistry will introduce students to the fundamental principles behind several basic and more advanced techniques commonly used in biochemical research. During the course, the strengths and weaknesses of each technique will be covered to explain which techniques are suitable for particular applications. | |||||
| Learning Outcomes | PO | MME | |
| The students who succeeded in this course: | |||
| LO-1 | can explain foundational biochemical laboratory methodology and techniques. |
PO-1 Upon successful completion of this programme, graduate students will: Have advance knowledge and skills in the area of specialized.
PO-2 Do enough practical and theoretical advance studies. PO-5 Follow the literature in the area of specialized. |
Examination Presentation |
| LO-2 | can develop the ability to design given logical experiments for a particular application, to perform these experiments successfully and independently, to interpret data appropriately, and to present data in clear writing. |
PO-3 Evaluate the scientific data obtained from advance studies and present scientific results. PO-4 Defend the thesis that in the front of a jury composed by experts. PO-7 Have enough knowledge of computer softwares used in Chemistry and use information and communication technologies in advance level. |
Examination |
| PO: Programme Outcomes MME:Method of measurement & Evaluation |
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| Course Contents | ||
| Lab introduction, safety and protocol writing, Buffer preparation, pH measurements Lecture, Spectrophotometer, Centrifugation , Protein purification, Gel-electrophoresis, Immunoblots and protein quantification, , Enzyme Kinetics, Recombinant DNA techniques, Mass spectrometry, Protein structure determination by X-ray crystallography | ||
| Weekly Course Content | ||
| Week | Subject | Learning Activities and Teaching Methods |
| 1 | Lab introduction, safety and protocol writing | Lecture, question and answer, discussion |
| 2 | Concentration calculations, Buffer preparation, pH measurements | Lecture, question and answer, discussion |
| 3 | Spectrophotometer | Lecture, question and answer, discussion |
| 4 | Centrifugation | Lecture, question and answer, discussion |
| 5 | Protein purification | Lecture, question and answer, discussion |
| 6 | Protein purification | Lecture, question and answer, discussion |
| 7 | Gel-electrophoresis | Lecture, question and answer, discussion |
| 8 | mid-term exam | |
| 9 | Immunoblots and protein quantification | Lecture, question and answer, discussion |
| 10 | Enzyme Kinetics | Lecture, question and answer, discussion |
| 11 | Enzyme Kinetics | Lecture, question and answer, discussion |
| 12 | Recombinant DNA techniques | Lecture, question and answer, discussion |
| 13 | Recombinant DNA techniques | Lecture, question and answer, discussion |
| 14 | Mass spectrometry | Lecture, question and answer, discussion |
| 15 | Protein structure determination by X-ray crystallography | Lecture, question and answer, discussion |
| 16 | final exam | |
| Recommend Course Book / Supplementary Book/Reading | ||
| 1 | Boyer, R.F., Modern Experimental Biochemistry, 3rd ed., Addison Wesley Pub. Co., 2000. | |
| 2 | Voet, D. and Voet, J.G., Biochemistry, 2nd edition, J. Wiley & Sons, N.Y., N.Y., 1995. | |
| Required Course instruments and materials | ||
| Lecture notes, books, related articles, computer | ||
| Assessment Methods | |||
| Type of Assessment | Week | Hours | Weight(%) |
| mid-term exam | 8 | 2 | 40 |
| Other assessment methods | |||
| 1.Oral Examination | |||
| 2.Quiz | |||
| 3.Laboratory exam | |||
| 4.Presentation | |||
| 5.Report | |||
| 6.Workshop | |||
| 7.Performance Project | |||
| 8.Term Paper | |||
| 9.Project | |||
| final exam | 16 | 2 | 60 |
| Student Work Load | |||
| Type of Work | Weekly Hours | Number of Weeks | Work Load |
| Weekly Course Hours (Theoretical+Practice) | 3 | 14 | 42 |
| Outside Class | |||
| a) Reading | 3 | 10 | 30 |
| b) Search in internet/Library | 3 | 14 | 42 |
| c) Performance Project | 0 | ||
| d) Prepare a workshop/Presentation/Report | 5 | 4 | 20 |
| e) Term paper/Project | 0 | ||
| Oral Examination | 0 | ||
| Quiz | 0 | ||
| Laboratory exam | 0 | ||
| Own study for mid-term exam | 3 | 6 | 18 |
| mid-term exam | 2 | 1 | 2 |
| Own study for final exam | 4 | 6 | 24 |
| final exam | 2 | 1 | 2 |
| 0 | |||
| 0 | |||
| Total work load; | 180 | ||