Nevşehir Hacı Bektaş Veli University Course Catalogue
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| Year/Semester of Study | 1 / Fall Semester | ||||
| Level of Course | 3rd Cycle Degree Programme | ||||
| Type of Course | Optional | ||||
| Department | MOLECULAR BIOLOGY AND GENETICS (PHD) | ||||
| Pre-requisities and Co-requisites | None | ||||
| Mode of Delivery | Face to Face | ||||
| Teaching Period | 14 Weeks | ||||
| Name of Lecturer | ÜMİT KUMBIÇAK (umitkumbicak@nevsehir.edu.tr) | ||||
| Name of Lecturer(s) | |||||
| Language of Instruction | Turkish | ||||
| Work Placement(s) | None | ||||
| Objectives of the Course | |||||
| The aim of this course is to gain knowledge and skills on the basic concepts of genotoxicology, the genotoxic effect mechanisms of environmental agents and current test methods used in the evaluation of genotoxic effects. | |||||
| Learning Outcomes | PO | MME | |
| The students who succeeded in this course: | |||
| LO-1 | can define the basic principles of genetic toxicology. |
PO-1 Have information about current issues in molecular biology and genetics. PO-2 Obtain basic knowledge of Molecular Biology PO-4 Evaluate the knowledge and skills in a critical way in every activity regarding the field, thereby guiding their learning |
Examination |
| LO-2 | can explain possible genotoxic effects mechanisms of physical, chemical and biological agents. |
PO-2 Obtain basic knowledge of Molecular Biology PO-4 Evaluate the knowledge and skills in a critical way in every activity regarding the field, thereby guiding their learning |
Examination |
| LO-3 | can explain the types of mutations. |
PO-1 Have information about current issues in molecular biology and genetics. PO-2 Obtain basic knowledge of Molecular Biology |
Examination |
| LO-4 | can define DNA repair mechanisms. |
PO-4 Evaluate the knowledge and skills in a critical way in every activity regarding the field, thereby guiding their learning PO-5 Being ethical and responsible individuals who are sensitive to social and universal values in the field of Molecular Biology and genetics, who look after the priority areas of the country, research and produce and contribute to these issues PO-6 Ability to design a research project in the field of molecular biology and genetics and to evaluate and present the results related to it |
Examination |
| LO-5 | can explain the mechanisms that cause carcinogenesis and teratogenesis. |
PO-1 Have information about current issues in molecular biology and genetics. PO-2 Obtain basic knowledge of Molecular Biology |
Examination |
| LO-6 | can describe current in vivo and in vitro genotoxicity tests in detail. |
PO-3 Make use of the knowledge they have in order to find a solution to problems by analyzing, comparing and questioning them and can carry out original research in the related field on their own PO-5 Being ethical and responsible individuals who are sensitive to social and universal values in the field of Molecular Biology and genetics, who look after the priority areas of the country, research and produce and contribute to these issues PO-6 Ability to design a research project in the field of molecular biology and genetics and to evaluate and present the results related to it |
Examination |
| PO: Programme Outcomes MME:Method of measurement & Evaluation |
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| Course Contents | ||
| Definition and scope of genetic toxicology, genotoxic effects and genotoxic action mechanisms of physical, chemical and biological agents, mutation types and formation mechanisms, DNA repair mechanisms, carcinogenesis, teratogenesis, an overview of in vivo and in vitro genotoxicity assays, Salmonella / Microsome Mutagenicity (AMES) assayt, Comet assay, Gamma H2AX assay, Sister Chromatid Exchange (SCE), Chromosome Abnormalities (KA), UDS (unscheduled DNA), Micronucleus (MN) assay, Allium assay, SMART assay and new genotoxicological test methods | ||
| Weekly Course Content | ||
| Week | Subject | Learning Activities and Teaching Methods |
| 1 | Genetic toxicology definition, scope and overview of in vivo and in vitro cytotoxicity and genotoxicity tests | Explanation Method, |
| 2 | Genotoxic physical, chemical and biological agents and their genotoxic action mechanisms and dose-time relationship | Explanation Method, |
| 3 | Mutation types and formation mechanisms, DNA repair mechanisms | Explanation Method, Article review |
| 4 | Expression analyzes of DNA damage signaling pathway and DNA repair genes by RT-PCR and UDS (unscheduled DNA) assay | Explanation Method, Article review |
| 5 | Use of PCR, RFLP and RAPD techniques to determine genotoxicity | Explanation Method, Article review |
| 6 | Cytotoxicity test methods, | Explanation Method, Article review |
| 7 | Salmonella / Microsome Mutagenicity (AMES) Assay, | Explanation Method, Article review |
| 8 | mid-term exam | |
| 9 | HPRT and XPRT gene mutation assays, Thymidine kinase (TK) gene mutation assay | Explanation Method, Article review |
| 10 | Comet assay, Gamma H2AX assay, | Explanation Method, Article review |
| 11 | Chromosome Abnormalities (CA) assay, Sister Chromatid Exchange (SCE) assay, | Explanation Method, Article review |
| 12 | Micronucleus (MN) assay, Allium assay, | Explanation Method, Article review |
| 13 | SMART assay | Explanation Method, Article review |
| 14 | In silico approaches for the determination of genotoxic effects | Explanation Method, Article review |
| 15 | Antigenotoxicity | Explanation Method, Article review |
| 16 | final exam | |
| Recommend Course Book / Supplementary Book/Reading | ||
| 1 | F. (Editor) Ünal And D. (Editor) Yüzbaşıoğlu. Genetic Toxicology. Ankara: Nobel Yayın Dağıtım 2022 | |
| Required Course instruments and materials | ||
| Course book, course presentations | ||
| Assessment Methods | |||
| Type of Assessment | Week | Hours | Weight(%) |
| mid-term exam | 8 | 1 | 40 |
| Other assessment methods | |||
| 1.Oral Examination | |||
| 2.Quiz | |||
| 3.Laboratory exam | |||
| 4.Presentation | |||
| 5.Report | |||
| 6.Workshop | |||
| 7.Performance Project | |||
| 8.Term Paper | |||
| 9.Project | |||
| final exam | 16 | 2 | 60 |
| Student Work Load | |||
| Type of Work | Weekly Hours | Number of Weeks | Work Load |
| Weekly Course Hours (Theoretical+Practice) | 3 | 14 | 42 |
| Outside Class | |||
| a) Reading | 5 | 8 | 40 |
| b) Search in internet/Library | 5 | 8 | 40 |
| c) Performance Project | 0 | ||
| d) Prepare a workshop/Presentation/Report | 0 | ||
| e) Term paper/Project | 0 | ||
| Oral Examination | 0 | ||
| Quiz | 0 | ||
| Laboratory exam | 0 | ||
| Own study for mid-term exam | 5 | 5 | 25 |
| mid-term exam | 1 | 1 | 1 |
| Own study for final exam | 5 | 6 | 30 |
| final exam | 2 | 1 | 2 |
| 0 | |||
| 0 | |||
| Total work load; | 180 | ||