Nevşehir Hacı Bektaş Veli University Course Catalogue

Information Of Programmes

FACULTY OF LETTERS & SCIENCE / KİM 383 - CHEMISTRY

Code: KİM 383 Course Title: CHROMATOGRAPHIC METHODS Theoretical+Practice: 2+0 ECTS: 4
Year/Semester of Study 3 / Fall Semester
Level of Course 1st Cycle Degree Programme
Type of Course Optional
Department CHEMISTRY
Pre-requisities and Co-requisites None
Mode of Delivery Face to Face
Teaching Period 14 Weeks
Name of Lecturer BAHAR TUBA FINDIK (btfindik@nevsehir.edu.tr)
Name of Lecturer(s)
Language of Instruction Turkish
Work Placement(s) None
Objectives of the Course
The aim of this course is to teach the basic principles of chromatography and basic concepts of chromatographic techniques.

Learning Outcomes PO MME
The students who succeeded in this course:
LO-1 can explain the basic concepts of chromatography.. PO-1 Comprehends the basic theory, laws and terms in the field of chemistry and can use the knowledge they have in the application areas related to the science of chemistry.
PO-3 Have the ability to use laboratory tools and materials effectively.
PO-13 Comprehend the contemporary techniques commonly used in chemistry and related fields and have the ability to use the modern devices required for the application of these techniques.
Examination
LO-2 Can identify the chromatographic methods for different applications. PO-3 Have the ability to use laboratory tools and materials effectively.
PO-4 Have the ability to conduct experiments, collect data, make qualitative and quantitative analysis, analyze data and design solution-oriented experiments, and analyze and interpret the results obtained.
PO-13 Comprehend the contemporary techniques commonly used in chemistry and related fields and have the ability to use the modern devices required for the application of these techniques.
Examination
LO-3 Can read and evaluate chromatographic data. PO-11 Can use information and communication technologies together with computer software at the level required by the field of chemistry, in this way, can reach the right scientific resources.
PO-12 By comprehending the importance of lifelong learning and following current innovations and developments in the field of chemistry, they gain the ability to continuously improve themselves.
PO-13 Comprehend the contemporary techniques commonly used in chemistry and related fields and have the ability to use the modern devices required for the application of these techniques.
Examination
PO: Programme Outcomes
MME:Method of measurement & Evaluation

Course Contents
Extraction, the theory of chromatography; adsorption and partion, chromatographic techniques; column chromatography, thin layer chromatography, paper chromatography, gas chromatography, high performance liquid chromatography (HPLC), ion exchange chromatography, electrophoresis, electrofocusing isotochophoresis, gel filtration and ultrafiltration.
Weekly Course Content
Week Subject Learning Activities and Teaching Methods
1 Introduction to chromatography, The theory of chromatography Lecture, question and answer, discussion
2 Chromatographic techniques Lecture, question and answer, discussion
3 Essential chromatographic parameters Lecture, question and answer, discussion
4 Problem-solving examples Lecture, question and answer, discussion
5 Thin layer chromatography Lecture, question and answer, discussion
6 Column chromatography Lecture, question and answer, discussion
7 Ion exchange chromatography Lecture, question and answer, discussion
8 mid-term exam
9 Reverse Phase/Normal Phase Chromatography Lecture, question and answer, discussion
10 Gas chromatography Lecture, question and answer, discussion
11 Gas chromatography Lecture, question and answer, discussion
12 High performance liquid chromatography (HPLC) Lecture, question and answer, discussion
13 High performance liquid chromatography (HPLC) Lecture, question and answer, discussion
14 Electrophoresis, electrofocusing isotochophoresis Lecture, question and answer, discussion
15 Gel filtration and ultrafiltration Lecture, question and answer, discussion
16 final exam
Recommend Course Book / Supplementary Book/Reading
1 Scott, R.P.W. (1995). Techniques and Practice of Chromatography (1st ed.). CRC Press, Boca Raton.
2 Nelson, David L., and Michael M. Cox. 2017. Lehninger Principles of Biochemistry. 7th ed. New York,
Required Course instruments and materials
Projector

Assessment Methods
Type of Assessment Week Hours Weight(%)
mid-term exam 8 1 40
Other assessment methods
1.Oral Examination
2.Quiz
3.Laboratory exam
4.Presentation
5.Report
6.Workshop
7.Performance Project
8.Term Paper
9.Project
final exam 16 2 60

Student Work Load
Type of Work Weekly Hours Number of Weeks Work Load
Weekly Course Hours (Theoretical+Practice) 2 14 28
Outside Class
       a) Reading 3 14 42
       b) Search in internet/Library 1 7 7
       c) Performance Project 0
       d) Prepare a workshop/Presentation/Report 0
       e) Term paper/Project 0
Oral Examination 0
Quiz 0
Laboratory exam 0
Own study for mid-term exam 5 4 20
mid-term exam 1 1 1
Own study for final exam 5 4 20
final exam 2 1 2
0
0
Total work load; 120