Nevşehir Hacı Bektaş Veli University Course Catalogue
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| Year/Semester of Study | 3 / Spring Semester | ||||
| Level of Course | 1st Cycle Degree Programme | ||||
| Type of Course | Optional | ||||
| Department | CHEMISTRY | ||||
| Pre-requisities and Co-requisites | None | ||||
| Mode of Delivery | Face to Face | ||||
| Teaching Period | 14 Weeks | ||||
| Name of Lecturer | BAHAR TUBA FINDIK (btfindik@nevsehir.edu.tr) | ||||
| Name of Lecturer(s) | |||||
| Language of Instruction | Turkish | ||||
| Work Placement(s) | None | ||||
| Objectives of the Course | |||||
| The aim of this course is to give information about biochemistry laboratory techniques and applications, to teach qualitative and quantitative analyses of protein, carbohydrate, lipid, vitamin and nucleic acids. | |||||
| Learning Outcomes | PO | MME | |
| The students who succeeded in this course: | |||
| LO-1 | can use biochemistry knowledge effectively in designing, analysing and interpreting experiments. |
PO-5 Gains the ability to establish interdisciplinary connections by combining different disciplines. PO-12 By comprehending the importance of lifelong learning and following current innovations and developments in the field of chemistry, they gain the ability to continuously improve themselves. PO-15 Health, environment, medicine, textile, food etc. solves unforeseen problems encountered in practice in industrial areas. |
Examination |
| LO-2 | can describe the appropriate methods used in the study of molecular structures. |
PO-3 Have the ability to use laboratory tools and materials effectively. PO-12 By comprehending the importance of lifelong learning and following current innovations and developments in the field of chemistry, they gain the ability to continuously improve themselves. PO-15 Health, environment, medicine, textile, food etc. solves unforeseen problems encountered in practice in industrial areas. |
Examination |
| LO-3 | can follow scientific developments in related fields. |
PO-5 Gains the ability to establish interdisciplinary connections by combining different disciplines. PO-12 By comprehending the importance of lifelong learning and following current innovations and developments in the field of chemistry, they gain the ability to continuously improve themselves. |
Examination |
| PO: Programme Outcomes MME:Method of measurement & Evaluation |
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| Course Contents | ||
| Examination of properties related to protein, carbohydrate, lipid, nucleic acid and vitamins. | ||
| Weekly Course Content | ||
| Week | Subject | Learning Activities and Teaching Methods |
| 1 | Buffers used in biochemistry and their preparation | Lecture, question and answer, discussion |
| 2 | Sample preparation techniques in biochemistry | Lecture, question and answer, discussion |
| 3 | Biochemical spectrophotometric and fluorometric determination methods | Lecture, question and answer, discussion |
| 4 | Extraction, pre-separation techniques | Lecture, question and answer, discussion |
| 5 | Disintegration processes by mechanical and chemical methods, homogenisation, solubilisation and clarification, | Lecture, question and answer, discussion |
| 6 | Centrifugation, microfiltration, ultrafiltration, lyophilisation | Lecture, question and answer, discussion |
| 7 | Dialysis, precipitation techniques (isoelectric precipitation, precipitation by ionic strength, precipitation by denaturation with organic solvents) | Lecture, question and answer, discussion |
| 8 | mid-term exam | |
| 9 | Types of chromatography, chromatographic methods, ion exchange, biochemical applications of gas and liquid chromatography | Lecture, question and answer, discussion |
| 10 | Ion exchange, gel filtration, hydrophobic interaction chromatography | Lecture, question and answer, discussion |
| 11 | Affinity, biospecific affinity, lectin affinity chromatography | Lecture, question and answer, discussion |
| 12 | Electrophoretic methods, principles of electrophoresis | Lecture, question and answer, discussion |
| 13 | PAGE, SDS-PAGE, 2D-PAGE, Imaging, DNA electrophoresis | Lecture, question and answer, discussion |
| 14 | Western ve Northern Blotlama, Elisa testleri, immünoblotlama | Lecture, question and answer, discussion |
| 15 | Application of HPLC | Lecture, question and answer, discussion |
| 16 | final exam | |
| Recommend Course Book / Supplementary Book/Reading | ||
| 1 | Telefoncu A., Salnıkow J., Zihnioğlu F. ve Kılınç A., 2000, Biyokimyada Temel ve Modern Teknikler, Kuşadası. | |
| 2 | Küfrevioğlu Ö. İ., Şakiroğlu H., Demir N., 1997 “Biyokimya Laboratuvar Ders Notları” Atatürk Üniversitesi Yayınları, Erzurum. | |
| 3 | Nelson, David L., and Michael M. Cox. 2017. Lehninger Principles of Biochemistry. 7th ed. New York, | |
| Required Course instruments and materials | ||
| Projector | ||
| Assessment Methods | |||
| Type of Assessment | Week | Hours | Weight(%) |
| mid-term exam | 8 | 1 | 40 |
| Other assessment methods | |||
| 1.Oral Examination | |||
| 2.Quiz | |||
| 3.Laboratory exam | |||
| 4.Presentation | |||
| 5.Report | |||
| 6.Workshop | |||
| 7.Performance Project | |||
| 8.Term Paper | |||
| 9.Project | |||
| final exam | 16 | 1 | 60 |
| Student Work Load | |||
| Type of Work | Weekly Hours | Number of Weeks | Work Load |
| Weekly Course Hours (Theoretical+Practice) | 2 | 14 | 28 |
| Outside Class | |||
| a) Reading | 4 | 14 | 56 |
| b) Search in internet/Library | 1 | 2 | 2 |
| c) Performance Project | 0 | ||
| d) Prepare a workshop/Presentation/Report | 0 | ||
| e) Term paper/Project | 0 | ||
| Oral Examination | 0 | ||
| Quiz | 0 | ||
| Laboratory exam | 0 | ||
| Own study for mid-term exam | 4 | 4 | 16 |
| mid-term exam | 1 | 1 | 1 |
| Own study for final exam | 4 | 4 | 16 |
| final exam | 1 | 1 | 1 |
| 0 | |||
| 0 | |||
| Total work load; | 120 | ||